The many other insects with a restricted diet, the citrus psyllid Diaphorina citri Kuwayama, the vector Causing Which agent of the HLB , the bacterium Candidatus spp Liberibacter., is intimately Associated with symbiotic microorganisms. These mutualistic symbionts play a key role on Their host nutritional ecology, and are vertically Transmitted to the progeny. However, Despite The role symbiont...

Mature mammalian eggs are ovulated arrested at meiotic metaphase II. Sperm break this arrest by an oscillatory Ca(2+) signal that is necessary and sufficient for the two immediate events of egg activation: cell cycle resumption and cortical granule release. Previous work has suggested that cell cycle resumption, but not cortical granule release, is mediated by calmodulin-dependent protein kinas...

Species' life history traits, including maturation age, number of reproductive bouts, offspring size and number, reflect adaptations to diverse biotic and abiotic selection pressures. A striking example of divergent life histories is the evolution of either iteroparity (breeding multiple times) or semelparity (breed once and die). We analysed published data on salmonid fishes and found that sem...

BACKGROUND The African clawed frog, Xenopus laevis, is widely used in studies of oogenesis, meiotic cell cycle and early embryonic development. However, in order to perform such studies, eggs are normally collected after the injection of hCG into the dorsal lymph sac of fully-grown female frogs following pre-injection of PMSF. Although this protocol is established and used as standard laborator...

Oocyte maturation in all species is controlled by a protein complex termed the maturation promoting factor (MPF). MPF comprises a cyclin-dependent kinase (CDK) and its partner cyclin, and it is regulated by dueling regulatory phosphorylation events on the CDK. In Caenorhabditis elegans, the Wee1/Myt1 ortholog WEE-1.3 provides the inhibitory phosphorylations on CDK-1 that keep MPF inactive and h...

HDAC8 is a class I histone deacetylase that functions in a variety of biological processes through its non-histone substrates. However, its roles during oocyte meiosis remain elusive. Here, we document that HDAC8 localizes at spindle poles and positively participates in the regulation of microtubule organization and spindle assembly in mouse oocytes. Depletion of HDAC8 by siRNA-based gene silen...

STUDY POPULATION. This study was a nonrandomized controlled, parallel-group intervention trial with an active study population of 50 consecutively enrolled children aged 5 to 18 years. The study children underwent egg OIT after confirming immunoglobulin (Ig)E-mediated egg allergy by using a double-blind, placebo-controlled egg food challenge (DBPCFC). The control group included 32 children who ...

Axolotl eggs were heat shocked (36-8°C, lOmin) inside their jelly layers. Heat shock (HS) was shown to induce the precocious appearance of a grey crescent (GC) in a number of eggs immediately after fertilization (Benford & Namenwirth, 1974). It was also demonstrated that this phenomenon occurs in fertilized or artificially activated eggs only when they are shocked within l jh after spawning. Th...