Systems biology proceeds through repeated cycles of experiment and modeling. One way to implement this is reverse engineering, where models are fit to data to infer and analyse regulatory mechanisms. This requires rigorous methods to determine whether model parameters can be properly identified. Applying such methods in a complex biological context remains challenging. We use reverse engineerin...

The bioactivity-guided fractionation of an active chloroform extract of Conyza albida led to the isolation of three alkenynes, deca-4,6-diyn-2-(Z)-enoic methyl ester (1), deca-4,6-diyn-2-(Z)-enoic ethyl ester (2) and deca-2,4-diene-4-hydroxy-6-yn-1,4-olide (3), and the terpenoid spathulenol (4), as the active toxic metabolites in the Artemia sp. lethality test. When tested in the KB cell cytoto...

Evidence for a persistent Na+ current (I(NaP)) in the apical dendrite of neocortical neurons was sought with the use of fluorescence imaging to measure changes in intradendritic Na+ concentration. Neurons in neocortical brain slices were filled iontophoretically through an intracellular recording microelectrode with the Na+-sensitive dye benzofuran isophthalate (SBFI), and fluorescence images w...

We mapped the sequence of ventricular depolarization in the crocodile Crocodylus johnstoni. We also attempted to find specialized conduction tissue within the ventricular myocardium. Electrical recordings with miniature multi-point electrodes revealed two strands of rapidly conducting tissue (channels) within the interventricular septum, suggestive of conductive tissue pathways. From these sept...

Det här är en undersökning som knyter an till en tidigare publicerad artikel om nitratfri luttring av ett soda-kalk-glas[1]. Det är ur miljösynpunkt bättre att använda antimonat som luttringsmedel efter som utsläppen av NOx-gaser blir betydligt mindre då inget nitrat tillsätts. Antimonat ger i soda-kalk-glaset lika god luttrande effekt som nitrat och antimonoxid. I den här artikeln har studien ...

There has been an apparent increase in the frequency and duration of El Niño–Southern Oscillation events in the last two decades relative to the prior period of record. Furthermore, 1990–95 was the longest period of sustained high Darwin sea level pressure in the instrumental record. Variations in the frequency and duration of such events are of considerable interest because of their implicatio...

We present a numerical study of parametrically driven surface waves in uids of low viscosity. The equations governing uid motion in the bulk and the appropriate boundary conditions at the free surface are approximated by a nonlocal set of equations involving surface coordinates alone, thus allowing the numerical analysis of the large aspect ratio limit (the aspect ratio being the ratio between ...

We mapped the sequence of ventricular depolarization in the crocodile Crocodylus johnstoni. We also attempted to find specialized conduction tissue within the ventricular myocardium. Electrical recordings with miniature multi-point electrodes revealed two strands of rapidly conducting tissue (channels) within the interventricular septum, suggestive of conductive tissue pathways. From these sept...

Pancreas transportation between donor center and islet production facility is frequently associated with prolonged ischemia impairing islet isolation and transplantation outcomes. It is foreseeable that shipment of pig pancreases from distant centralized biosecure breeding facilities to institutes that have a long-term experience in porcine islet isolation is essentially required in future clin...

UNLABELLED Superinfection exclusion (SIE), the ability of an established virus infection to interfere with a secondary infection by the same or a closely related virus, has been described for different viruses, including important pathogens of humans, animals, and plants. Citrus tristeza virus (CTV), a positive-sense RNA virus, represents a valuable model system for studying SIE due to the exis...