The use of doubled haploids in onion breeding is limited due to the low gynogenesis efficiency of this species. Gynogenesis capacity from Spanish germplasm, including the sweet cultivar Fuentes de Ebro, the highly pungent landrace BGHZ1354 and the two Valenciana type commercial varieties Recas and Rita, was evaluated and optimized in this study. The OH-1 population, characterized by a high gyno...

Reproductive stage frost poses a major constraint for wheat production in countries such as Australia. However, little progress has been made identifying key genes to overcome the constraint. In present study, severe event hit two large-scale field trials consisting of six doubled haploid (DH) populations at reproductive (young microspore stage) Western Australia, leading identification 30 robu...

For about a century, researchers have been working on haploidy approach. Progressively, they discovered the importance and usefulness of haploids in various research fields. On other hand, it is suggested that climate change impacts crop production, especially wheat, requires rapid efficient methods plant breeding to produce new cultivars with sufficient level biotic abiotic tolerance avoid sig...

an efficient method for producing doubled haploid plants of date palm ( phoenix dactylifera l.) was established using in vitro colchicine treatment of anthers. to this study male inflorescences were harvested in the appropriate developmental stage. separated anthers after one day cold (4°c) pretreatment, in uninucleate stage of microspore were transferred to the modified y 3 medium containing d...

Assessment of genetic purity parental inbred lines and their resultant F 1 hybrids is an essential quality control check in maize hybrid breeding, variety release seed production. In this study, purity, parent-offspring relationship diversity among the were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total 188 genotypes, comprising 26 lines, four doubled haploid (DH) 158 s...

Majority of higher plants are outbreeding, highly heterozygous and undergo a long developmental period before reaching their reproductive stage. Traditional breeding and crosspollinating procedures are both unpredictable and time-consuming. Haploids are plants with a gametophytic chromosome number and doubled haploids are haploids that have undergone chromosome duplication, represent a particul...

Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3 (-∕-) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after indep...

Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, and flow cytometry techniques. Evaluated...