The isolated perfused heart system, as originated by Oscar Langendorff more than a century ago, has become a predominant technique in pharmacological and physiological research. The technique allows the examination of cardiac contractile strength (inotropic effects), heart rate (chronotropic effects) and vascular effects without the complications of an intact animal model. From its simple begin...

BACKGROUND Victims of severe accidental hypothermia are prone to fluid extravasation but rarely develop lung edema. We hypothesize that combined hypothermia-induced increase in pulmonary vascular resistance (PVR) and a concomitant fall in cardiac output protect the lungs against edema development. Our aim was to explore in hypothermic-isolated blood-perfused rat lungs whether perfusion at const...

D. A. RANDALL, l R. D. CESS, 2 J.P. BLANCHET, 3 G. J. BOER, 3 D. A. DAZLICH, • A.D. DEL GENIO, 4 M. DEQUE, 5 V. DYMNIKOV, 6 V. GALIN, 6 S. J. GHAN, 7 A. A. LACIS, 4 H. LE TREUT, 8 Z.-X. LI, 8 X.-Z. LIANG, 9 B. J. MCAVANEY, •ø V. P. MELESHKO, TM J. F. B. MITCHELL, •2 J.-J. MORCRETTE, •3 G. L. POTTER, 7 L. RIKUS, lø E. ROECKNER, TM J. F. ROYER, 5 U. SCHLESE, TM D. m. SHEININ, TM J. SLINGO, 15 A. ...

Isolated hearts subjected to ischemia-reperfusion (I/R) exhibit depressed cardiac performance and alterations in subcellular function. Since hearts perfused at constant flow (CF) and constant pressure (CP) show differences in their contractile response to I/R, this study was undertaken to examine mechanisms responsible for these I/R-induced alterations in CF-perfused and CP-perfused hearts. Rat...

It is unknown whether ventricular fibrillation (VF) studied in experimental models represents in vivo human VF. First, we examined closed chest in vivo VF induced at defibrillation threshold testing (DFT) in four patients with ischemic cardiomyopathy pretransplantation. We examined VF in these same four hearts in an ex vivo human Langendorff posttransplantation. VF from DFT was compared with VF...

BACKGROUND Previous studies have suggested that pulmonary embolism (PE) and pulmonary artery occlusion result in a shift in alveolar ventilation away from unperfused regions. This study aimed to directly assess changes in regional specific ventilation (sV(A)) due to autologous blood clot PE using positron emission tomography. METHODS Pulmonary embolism was created in six anesthetized, paralyz...

In the current study, effects of acute short term administration of fructose on cardiac arrhythmias and myocardial infarction size following ischemia/reperfusion were investigated in isolated rat heart. The hearts were subjected to 30 min zero flow global ischemia followed by 120 min reperfusion. In the control group, the hearts were perfused by normal drug free Krebs-Henseleit (K/H) solution t...

To evaluate the reliability of using in situ frog intestinal perfusion technique for permeability assessment of carrier transported drugs which are also substrates for CYP enzymes. Single Pass Intestinal Perfusion (SPIP) studies were performed in frogs of the species Rana tigrina using established method for rats with some modifications after inducing anesthesia. Effective permeability coeffici...