Efficacy of primers capable of amplifying conserved outer membrane protein (OMP) genes i.e., lipL21 and lipL32 of Leptospira strains was tested for rapid and early diagnosis of the leptospirosis using a polymerase chain reaction (PCR). These OMP genes were found to be conserved in various leptospiral serovars viz., Canicola, Pomona, Icterohaemorrhagiae, Pyrogenes, Sejroe, Grippotyphosa, Ballum ...

Rabbit lymphosarcoma tissues contain 70 S RNA and RNA-directed DNA polymerase encapsulated in particulate components that band in the density region of type C RNA viruses. RNA-directed DNA polymerase associated with the particles could be distinguished from cellular DNA polymerases by salt elution from phosphocellulose. The enzyme preferred the template primers poly(rA)-(dT)12-18 and poly(rC)-(...

Efficient method for isolation of high quality genomic DNA is the first step in the development of DNA based markers for fingerprinting and genetic diversity of crops. We have described a simple and user friendly protocol for extraction of DNA from dried sugarcane leaf samples, which dose not require any use of liquid nitrogen, making it advantageous over other common protocols. The DNA obtaine...

NY-ESO-1 is a cancer/testis (CT) antigen expressed in normal adult tissues solely in the testicular germ cells of normal adults and in various cancers. It induces specific humoral and cellular immunity in patients with NY-ESO-1-expressing cancer. We compared the expression of NY-ESO-1 mRNA in hepatocellular carcinoma (HCC) patients by using various primers and DNA polymerases to optimize RT-PCR...

Leishmania infantum causes visceral leishmaniasis (VL) in the New World. The diagnosis of VL is confirmed by parasitological and serological tests, which are not always sensitive or specific. Our aim was to design new primers to perform a Polymerase Chain Reaction (PCR) for detecting L. infantum. Sequences of the minicircle kinetoplast DNA (kDNA) were obtained from GenBank, and the FLC2/RLC2 pr...

Three Malaysian ginger cultivars (Bukit Tinggi, Tanjung Sepat and Sabah) were collected and examined for genetic polymorphisms using microsatellite DNA primers. The single microsatellite oligonucleotide primers (CATA)5, (GATA)5 and (GAC)6 were used in polymerase chain reactions (PCRs). These PCR reactions produced 7 polymorphic bands with an average of 2.334 polymorphic bands per primer, leadin...

A multi-primer PCR system with an open architecture has been developed for rapid detection of bacterial and viral pneumonia pathogens. The design methodology includes the study several key biophysical physico-chemical parameters primers to ensure their compatibility in multiplex PCR. Due compatibility, can be expanded if necessary by adding compatible specific other or agents, which include bot...

The lagging strand of the replication fork is initially copied as short Okazaki fragments produced by the coupled activities of two template-dependent enzymes, a primase that synthesizes RNA primers and a DNA polymerase that elongates them. Gene 4 of bacteriophage T7 encodes a bifunctional primase-helicase that assembles into a ring-shaped hexamer with both DNA unwinding and primer synthesis ac...

It has been suggested that two distinct species exist within what was originally known as Entamoeba histolytica and E. dispar. These are Entamoeba dispar for the nonpathogenic and E. histolytica for the pathogenic fonn. Differentiation of these two organisms is of great clinical importance since they are morphologically indistinguishable and both fonns can infect the human intestinal cavity...

The lethal gene 'Luteus-Pa' is found in cacao genotypes (Theobroma cacao) of the Parinari (Pa) series, from Peru. Seedlings affected by this gene have yellowing leaves and subsequently die. We mapped this gene based on microsatellite markers and RAPDs, in order to elucidate the inheritance of 'Luteus-Pa' and investigate possible lethal mechanisms. DNA samples of genitors were amplified with 87 ...