Microbes in nature are rarely amenable to growth by standard microbiological methods, with the majority being unculturable. Metagenomic methods help to bypass and overcome the limitations of traditional culturing method; wherein total community DNA is isolated, cloned into suitable vector and host systems. However, isolation of total community DNA itself remains a challenge. In this study five ...

The isolation of intact, high-molecular mass genomic DNA is essential for many molecular biology applications including PCR, endonuclease digestion, southern blot analysis, and genomic library construction. Many protocols are available for the extraction of genomic DNA from plant tissue, and all the protocols have used expensive and hazardous chemical-liquid nitrogen for grinding plant material...