in this study, the four cr-mn-(ni-free) austenitic steels were fabricated by vacuum induction furnace. then plates with 10 mm thickness were fabricated by hot-rolling. corrosion behaviour of the four cr-mn steels in 0.1 m h2so4 solution was investigated using by open-circuit potential, potentiodynamic polarization, and electrochemical impedance spectroscopy (eis). the results indicated that the...

Realization of a novel single-resistance-controlled oscillator, employing an active element and all grounded passive elements, is the purpose of this manuscript. With requirements for completing the design being only a single Voltage Differencing Current Conveyor and four grounded passive components, it is also a preferable choice for integrated circuit implementation. The designed circuit has ...

A simple and enzyme-free strip biosensor for the amplified detection of Pb(2+) has been constructed based on a catalytic DNA circuit. This assay is ultrasensitive, enabling the visual detection of Pb(2+) concentrations as low as 10 pM without instrumentation.

Gene regulatory circuits can receive multiple simultaneous inputs, which can enter the system through different locations. It is thus necessary to establish how these genetic circuits integrate multiple inputs as a function of their relative entry points. Here, we use the dynamic circuit regulating competence for DNA uptake in Bacillus subtilis as a model system to investigate this issue. Speci...

We demonstrate a strategy to trigger and finely control the assembly of supramolecular DNA nanostructures with pH. Control is achieved via a rationally designed strand displacement circuit that responds to pH and activates a downstream DNA tile self-assembly process. We observe that the DNA structures form under neutral/basic conditions, while the self-assembly process is suppressed under acidi...

Monolayers of cysteine linked peptide nucleic acid (PNA) assembled on gold electrodes were investigated for the sensing of DNA recognition. The monolayer was characterized by cyclic voltammetry (CV). The electron transfer through the monolayers was investigated using electrochemical impedance spectroscopy (EIS) in the presence of target DNA and redox marker ions [Fe(CN)6] 3 /4 as hybridization ...

p53 plays a pivotal role in tumour suppression under stresses, such as DNA damage. ISG15 has been implicated in the control of tumorigenesis. Intriguingly, the expression of ISG15, UBE1L and UBCH8 is induced by DNA-damaging agents, such as ultraviolet and doxorubicin, which are known to induce p53. Here, we show that the genes encoding ISG15, UBE1L, UBCH8 and EFP, have the p53-responsive elemen...

Biomolecular interactions have important cellular implications, however, a simple method for the sensing of such proximal events is lacking in the current molecular toolbox. We designed a dynamic DNA circuit capable of recognizing targets in close proximity to initiate a pre-programmed signal transduction process resulting in localized signal amplification. The entire circuit was engineered to ...

This work presents miniaturized CMOS (complementary metal oxide semiconductor) sensors for non-faradic impedimetric detection of AIV (avian influenza virus) oligonucleotides. The signal-to-noise ratio is significantly improved by monolithic sensor integration to reduce the effect of parasitic capacitances. The use of sub-μm interdigitated microelectrodes is also beneficial for promoting the sig...

Mu DNA transposition proceeds through a series of higher-order nucleoprotein complexes called transpososomes. The structural core of the transpososome is a tetramer of the transposase, Mu A, bound to the two transposon ends. High-resolution structural analysis of the intact transposase and the transpososome has not been successful to date. Here we report the structure of Mu A at 16-angstroms an...