Addition of dihydro-N-demethylstreptomycin (DH-NDMS)or N-demethylstreptomycin (NDMS)in a concentration of lOmg/ml to a growing culture of S. griseus blocked the de novo biosynthesis of streptomycin (SM). A simultaneous addition of 14CH3-L-methionine, however, led to specific N-methylation of the added compounds, thus giving the authentic antibiotics dihydrostreptomycin (DHS) and SM. By using th...

Clinically suspected hypomagnesaemia was confirmed in 21 patients over 12 months; all patients had been exposed to either short-term vigorous diuretic treatment or moderate-dosage long-term treatment. Magnesium depletion was compounded by a hospital diet surprisingly low in magnesium, a local soft water supply, and, in some patients, high alcohol intake. Common presenting symptoms included depr...

Diuretics are pharmacological agents that increase natriuresis through inhibition of tubular re-absorption of sodium. The mechanisms and site of this inhibition differ with each drug class, accounting for their additive effects on natriuresis increase and their hydroelectrolytic side effects. The response to a given diuretic dose depends on the diuretic concentration on the urine at its action ...