Guava (Psidium guajava L.) is a highly perishable fruit crop comparable to mango owing to its high medicinal value and intense aroma. The presence of high genetic variability limits the chances of further expansion of guava improvement using biotechnological interventions. Conventional methods of guava improvement encountered with restricted achievement in progress of disease resistant varietie...

Somatic embryogenesis is a developmental process during which plant somatic cells, under in vitro conditions, produce embryogenic cells that develop into embryos. an efficient method clonal propagation of plants. Mature embryos were used as explant source for callus formation and the produced compact, healthy most mature induced callus. showed similar morphologically to their non-somatic counte...

Somatic embryogenesis (SE) is the developmental restructuring of somatic cells towards the embryogenic pathway and forms the basis of cellular totipotency in angiosperms. With the availability of full-length cDNA sequences from Knowledge-based Oryza Molecular Biological Encylopedia (KOME), we identified the leucine-rich repeat receptor-like kinase (LRR-RLK) genes from rice (Oryza sativa), which...

Plant embryogenesis is a fascinating developmental program that is very successfully established in nature in seeds. In case of in vitro somatic embryogenesis this process is subjected to several limitations such as asynchronous differentiation and further development of somatic embryos, malformations and disturbed polarity, precocious germination, lack of maturity, early loss of embryogenic po...

this review article investigates a comprehensive description of the factors, methods, strategies, approaches and prerequisites influencing in vitro micropaopagation and the amount of their use across the world. the findings presented here are the results of the study of several hundred papers published all over the world. tissue culture techniques are routinely used for micropropagation. enhanc...

BACKGROUND The plant cell walls play an important role in somatic embryogenesis and plant development. Pectins are major chemical components of primary cell walls while homogalacturonan (HG) is the most abundant pectin polysaccharide. Developmental regulation of HG methyl-esterification degree is important for cell adhesion, division and expansion, and in general for proper organ and plant deve...

An efficient embryogenic response (both direct and indirect) of two different varieties of sugarcane (CP 77,400 and BL-4) was obtained in this study. The results demonstrates that fresh inner whorl of leaves and shoot apical meristem of sugarcane were highly amenable to in vitro somatic embryogenesis (both direct and indirect). A combination of 3.0 mg/l 2,4-D with 0.25 mg/l BAP was more potent ...

Nanos (Nos) is one of the evolutionarily conserved proteins known to direct germ-line development. In Drosophila, maternal Nos protein maintains transcriptional quiescence in the germ-line progenitors or pole cells to repress ectopic expression of somatic genes. Here we show that maternal Nos is required to establish and maintain germ-line identity by preventing apoptosis and somatic cell fate....

The induction and control of in vitro somatic embryogenesis is dependent of a number of factors. The genotype of the mother plant donor of explants and the kind of auxin play essential roles in conferring embryogenic competence. Once obtained somatic embryos may be encapsulated in alginate originating synthetic seeds that can be stored at low temperatures. Considering this the present work aime...