Flavin-containing monooxygenases (FMO) catalyze the oxidation of certain xenobiotics and drugs which contain a nucleophilic heteroatom. Here we report the first assessment of human brain flavin-containing monooxygenase from tissues obtained at autopsy from seven traffic accident victims. Human brain microsomes catalyzed the S-oxidation or N-oxidation of model substrates methimazole and N,N-dime...

We have evaluated the Technicon SMA 12/60 modification of a glucose oxidase/peroxidase-3-methyl2-benzothiazolinone hydrazone-N, N-dimethylaniline method, and find it acceptable. Added bilirubin, creatinine, dextrans, and uric acid did not interfere. We compared values for patients’ sera by this method to those by the glucose oxidase/peroxidase-2,2’-azinodiethylbenzothiazoline-6-sulfonic acid, n...

We evaluated the analytical performance of Trinder's glucose oxidase (EC 1.1.3.4)/peroxidase (EC 1.11.1.7) 4-aminophenazone-phenol method for the quantification of serum glucose as adapted to the Technicon SMAC. Our results correlated well with those by the routine SMAC glucose oxidase/peroxidase 3-methyl-2-benzothiazolinone hydrazone-N,N-dimethylaniline method (y = 1.02x - 49.4; r = 0.99) and ...

Femtosecond time-resolved infrared spectroscopy was employed to study intramolecular charge transfer in triphenylmethane dyes, including malachite green (MG), malachite green carbinol base (MGCB), and leucomalachite green (LMG). A local excited state (LE) and a twisted intramolecular charge-transfer (TICT) state have been observed directly in MG. Furthermore, solvent-controlled TICT measurement...

Molecular structures of 4-(((4-methoxyphenyl)amino)methyl)-N,N-dimethylaniline and 2-methoxy-5-((phenylamino)methyl)phenol synthesized via Schiff bases reduction route are reported. The compounds consist asymmetric units C16H20N2O (1) C14H15NO2 (2) in orthorhombic monoclinic crystal systems, respectively. Compound 1 intermolecular C11—H11···N2 hydrogen bonding with C11···N21 = 3.463(4) Å. hydro...