The degradation of chondroitin 4-[35S]sulphate isolated from chick-embryo cartilage was studied in the rat by experiments on free-range animals, on wholly anaesthetized animals with ureter cannulae, by perfusion of isolated liver, by whole-body radioautography and by isolation of liver lysosomes. After injection into rats 68% of the radioactivity was recovered in the urine after 24h, approximat...

Nickel sulphate more frequently determines allergic dermatitis due to contact (contact eczema); less known are nasal inflammation (rhinitis) and bronchial asthma caused by nickel sulphate. Sporadic cases, often related to patients' work have been reported in the literature. The research described herein refers to 20 patients presenting clear nickel allergy with rhinitis (associated in 11 cases ...

Two simple methods for dissolving salts of acid glycosaminoglycans with inorganic cations (e.g. Li+ and Na+) in dry dimethyl sulphoxide are described. Complete n.m.r. spectra of, e.g., Na+ and Li+ salts of chondroitin sulphate and keratan sulphate were obtained on these solutions. In [2H6]dimethyl sulphoxide the NH resonance of 2-acetamido-2-deoxy hexosides is in the range 7.2-8.0 delta, but is...

In human hepatoma HepG2 cell line curdlan sulphate enhances basal and interleukin-6-stimulated fibrinogen and antichymotrypsin (ACT) synthesis, slightly increases basal ceruloplasmin production and exerts only minor effects on alpha-1-proteinase inhibitor and transferrin. Curdlan sulphate may, at least in part, affect protein synthesis at a pretranslational level, as the expression of ACT mRNA ...

1. Selenate uptake by human placental brush-border-membrane vesicles was studied in order to establish whether this anion shares a pathway with sulphate. 2. Selenate uptake was found to be saturable with respect to medium selenate and was inhibited by the anion exchange inhibitor 4,4'-diisothiocyano-stilbene-2,2'-disulphonate (DIDS). 3. Anions which have a similar tetrahedral shape to selenate,...

Synthesis of sulphated proteoglycans by cell lines derived from explants of 7 rheumatoid and 9 normal specimens of synovial tissue, as well as by 7 lines of skin fibroblasts from non-rheumatoid patients, was examined. Cells of all 3 types were cultured as monolayers. They were then disaggregated and their capacity to synthesise proteoglycan estimated in cell suspensions by the incorporation of ...

Foetal human lung fibroblasts, grown in monolayer, were allowed to incorporate 35SO42for various periods of time. 35S-labelled macromolecular anionic products were isolated from the medium, a trypsin digest of the cells in monolayer and the cell residue. The various radioactive polysaccharides were identified as heparan sulphate and a galactosaminoglycan population (chondroitin sulphate and der...

We have previously shown that a sulphate activating system is present on the outside of the inner mitochondrial membrane of Euglena gracilis Klebs. var. bacillaris Cori, but efforts to couple this system to ATP produced from oxidative phosphorylation were unsuccessful. In the present work we show that the concentration of Pi ordinarily used to support oxidative phosphorylation in these mitochon...

Sulphate leaching losses may reduce the long-term possibility of maintaining the S supply of crops in low input farming systems. Sulphate leaching and S balances were investigated in an organic dairy/crop rotation (barley [Hordeum vulgare] → grass-clover [Lolium perenne/Trifolium repens] → grass-clover → barley/pea [Pisum arvénse] → winter wheat [Tritucum aestivum] → fodder beet [Beta vulgaris]...

Between Days 9 and 15 after oestrus, concentrations of pregnenolone, pregnenolone sulphate, dehydroepiandrosterone (DHEA), DHEA sulphate, androstenedione, oestrone and oestrone sulphate in free uterine fluid collected from non-pregnant gilts were greater than respective values in plasma (P less than 0.05). The total contents of pregnenolone, progesterone, DHEA, testosterone, oestrone and oestra...