نتایج جستجو برای: dichlorofluorescein

تعداد نتایج: 578  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1998
H D Teixeira R I Schumacher R Meneghini

Transfection of V79 Chinese hamster cells produced clones in which CuZn-superoxide dismutase (CuZn-SOD) activities were 2.2- to 3. 5-fold higher than in the parental cells. An overall reduction of antioxidant enzyme activities and both total and oxidized glutathione levels had been found in these clones. Aconitase activities in these cells were determined to indirectly measure the O2- steady-st...

2011
Ismail Syed Chandrashekara N. Kyathanahalli Bhavaani Jayaram Sudha Govind Christopher J. Rhodes Renu A. Kowluru Anjaneyulu Kowluru

OBJECTIVE To determine the subunit expression and functional activation of phagocyte-like NADPH oxidase (Nox), reactive oxygen species (ROS) generation and caspase-3 activation in the Zucker diabetic fatty (ZDF) rat and diabetic human islets. RESEARCH DESIGN AND METHODS Expression of core components of Nox was quantitated by Western blotting and densitometry. ROS levels were quantitated by th...

2014
Chang-Suk Kong

As a part of ongoing research to elucidate and characterize antioxidant and anti-inflammatory nutraceuticals, solvent-partitioned fractions from Spergularia marina were tested for their ability to scavenge radicals and suppress inflammation. The results of the 2',7'-dichlorofluorescein diacetate assay indicate that solvent-partitioned fractions from S. marina scavenged intracellular radicals in...

Journal: :Investigative ophthalmology & visual science 2006
Mélody Dutot François Pouzaud Isabelle Larosche Françoise Brignole-Baudouin Jean-Michel Warnet Patrice Rat

PURPOSE To investigate in vitro whether eye toxicity is attributable to the preservative or the fluoroquinolone used in ophthalmic formulations. METHODS Corneal and conjunctival cell lines were incubated with preserved (benzalkonium chloride [BAC]) or unpreserved ofloxacin solutions for 15 minutes. Several concentrations of BAC were also tested (0.0025%-0.01%). Membrane integrity, reactive ox...

2015
Jaya Singhal Surinder P. Singh Stalin Karuppiah Alok K. Pandey

Unique physicochemical properties of carbon nanomaterials (CNMs) have opened a new era for therapeutics and diagnosis (known as theranostics) of various diseases. This exponential increase in application makes them important for toxicology studies. The present study was aimed at exploring the toxic potential of one of the CNMs, that is, bucky tubes (BTs), in human lung adenocarcinoma (A549) cel...

Journal: :Biological & pharmaceutical bulletin 2009
Xiaomei Zhang Jun Cao Liping Jiang Laifu Zhong

This study was designed to investigate whether hydroxytyrosol (HT) may ameliorate oxidative stress and nuclear factor kappaB (NF-kappaB) activation in the lipopolysaccharide (LPS)-stimulated THP-1 cell line. We measured the intracellular reactive oxygen species (ROS) formation using 2,7-dichlorofluorescein diacetate (DCFH-DA) as a fluorescent probe. Intracellular glutathione (GSH) level was est...

Journal: :The American journal of pathology 2006
Azza B El-Remessy Mohamed Al-Shabrawey Yousuf Khalifa Nai-Tse Tsai Ruth B Caldwell Gregory I Liou

Diabetic retinopathy is characterized by blood-retinal barrier (BRB) breakdown and neurotoxicity. These pathologies have been associated with oxidative stress and proinflammatory cytokines, which may operate by activating their downstream target p38 MAP kinase. In the present study, the protective effects of a nonpsychotropic cannabinoid, cannabidiol (CBD), were examined in streptozotocin-induc...

2013
Elhaseen Elamin Ad Masclee Kati Juuti-Uusitalo Sven van IJzendoorn Freddy Troost Harm-Jan Pieters Jan Dekker Daisy Jonkers

BACKGROUND & AIMS Evidence is accumulating that ethanol and its oxidative metabolite, acetaldehyde, can disrupt intestinal epithelial integrity, an important factor contributing to ethanol-induced liver injury. However, ethanol can also be metabolized non-oxidatively generating phosphatidylethanol and fatty acid ethyl esters (FAEEs). This study aims to investigate the effects of FAEEs on barrie...

Journal: :Circulation research 2000
T Vanden Hoek L B Becker Z H Shao C Q Li P T Schumacker

Cardiomyocyte death after ischemia/reperfusion correlates with oxidant stress, and antioxidants confer protection in that model. Preconditioning (PC) with hypoxia or adenosine also confers protection, leading us to hypothesize that PC protects by attenuating oxidant generation during subsequent ischemia/reperfusion. Chick cardiomyocytes were preconditioned with 10 minutes of hypoxia or adenosin...

2016
Naser Abbasi Afra Khosravi Ali Aidy Massoumeh Shafiei

BACKGROUND Clinical evidence indicates the diabetes-induced impairment of osteogenesis caused by a decrease in osteoblast activity. Flavonoids can increase the differentiation and mineralization of osteoblasts in a high-glucose state. However, some flavonoids such as luteolin may have the potential to induce cytotoxicity in osteoblast-like cells. This study was performed to investigate whether ...

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