The dextran sulphates resemble heparin in being active inhibitors of several different forms of biological activity, e.g., blood coagulation, serum complement activity, and certain enzyme systems (Walton, 1954). In each case the activity of the dextran sulphate appears to be dependent upon direct interaction with one or more protein components of the biological system under investigation. In fa...

The enzyme-linked immunosorbent assay (ELISA) is a potential tool for the determination of dextran. In this study, dextran neoglycoprotein antigens were prepared by Reductive Amination method, and were confirmed by SDS-PAGE and free amino detection. The impact factors such as different oxidation degree of dextran, the conjugate reaction time to BSA were investigated. The best preparation condit...

Casein hydrolysis was carried out in PEG-dextran two-phase system by using a-chymotrypsin immobilized on a water soluble polymer Eudragit S-100. This biocatalyst along with the substrate casein was predominantly found to partition into the PEG phase. Under these optimized conditions, the product was found to partition (84%) in the dextran phase. Removal of dextran phase at an appropriate time i...

The bioavailability of ketoprofen after oral administration of aqueous solutions of various ketoprofen-dextran ester prodrugs in pigs was assessed. Conjugates derived from dextran fractions in the molecular weight range 10,000-500,000 were employed. Compared to the administration of an oral solution of an equivalent dose of parent ketoprofen, the average absorption fractions for the different p...

This report continues or examination of the effect of genetically engineered charge modifications on the partitioning behavior of proteins in aqueous two-phase extration. The genetic modifications consisted of the fusion of charged peptide tails to beta-galactosidase and charge-change point mutations to T4 lysozyme. Our previous article examined the influence of these charge modifications on pa...

Precipitated calcium carbonate (PCC) was prepared by means of semicontinuous carbonation of Ca(OH)2 suspension, at 35 and 45 °C and in the presence of non-ionic dextran and dextran sulfate. The carbonation process was regulated at different predetermined values of electrical conductivity, that corresponded to different concentrations of dissolved Ca(OH)2: 0.5 mS cm < κ25 < 5.0 mS cm. The result...

Proteins function in cellular environments that are crowded with biomolecules, and in this reduced available space, their biophysical properties may differ from those observed in dilute solutions in vitro. Here, we investigated the effects of a synthetic macromolecular crowding agent, dextran 20, on the folded states of hyperthermophilic (S16Thermo) and mesophilic (S16Meso) homologs of the ribo...

The use of grains as an alternative to wheat in breadmaking has rapidly grown the last few years, driven by Sustainable Development Goals toward improving food security and promoting sustainable agriculture. Flours from legumes, pseudo-cereals, minor cereals milling by-products, such bran, are particular interest. production partially substituted or wheat-free bread is, however, a challenging t...