Slp4-a (synaptotagmin-like protein 4-a)/granuphilin-a is specifically localized on dense-core vesicles in PC12 cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A via the N-terminal Slp homology domain (SHD) (Fukuda, M., Kanno, E., Saegusa, C., Ogata, Y., and Kuroda, T. S. (2002) J. Biol. Chem. 277, 39673-39678). However, the mechanism of the inh...

Dense core vesicles (DCVs) are thought to be generated at the late Golgi apparatus as immature DCVs, which subsequently undergo a maturation process through clathrin-mediated membrane remodeling events. This maturation process is required for efficient processing of neuropeptides within DCVs and for removal of factors that would otherwise interfere with DCV release. Previously, we have shown th...

INTRODUCTION Dense granule (DG) deficiency (DGD) is a feature of some platelet function disorders (PFD) with a prevalence similar to von Willebrand disease. Most laboratories assess for DGD using whole mount platelet preparations and electron microscopy (EM). We evaluated our experiences with this test and associations between DGD and bleeding. METHODS Dense granule EM records for 2006-2017 w...

In this paper we study how to compute a dense depth map with panoramic field of view (e.g., 360 degrees) from multiperspective panoramas. A dense sequence of multiperspective panoramas is used for better accuracy and reduced ambiguity by taking advantage of significant data redundancy. To speed up the reconstruction, we derive an approximate epipolar plane image that is associated with the plan...

The exploration of the phase diagram of dense baryonic matter is an area of intense theoretical and experimental activity. Baryonic systems, from dilute neutron matter at low density to superconducting quark matter at high density, exhibit an enormous variety of many-body effects. Despite its simplicity all these phenomena are ultimately described by the lagrangian of QCD. In practice it is usu...

This paper proposes a pooling strategy for local descriptors to produce a vector representation that is orientation-invariant yet implicitly incorporates the relative angles between features measured by their dominant orientation. This pooling is associated with a similarity metric that ensures that all the features have undergone a comparable rotation. This approach is especially effective whe...

Mepacrine uptake into platelets and bone marrow megakaryocytes was analyzed to further characterize the dense granule defects in a group of seven mouse pigment mutants that have characteristics of platelet storage pool disease (SPD). In contrast to our previous studies using electron microscopy, this method revealed that all mutants had normal numbers of dense granules. However, total mepacrine...