Acetylation is frequently detected on mitochondrial enzymes, and the sirtuin deacetylase SIRT3 is thought to regulate metabolism by deacetylating mitochondrial proteins. However, the stoichiometry of acetylation has not been studied and is important for understanding whether SIRT3 regulates or suppresses acetylation. Using quantitative mass spectrometry, we measured acetylation stoichiometry in...

Activation-induced Fas ligand (FasL) mRNA expression in CD4+ T cells is mainly controlled at transcriptional initiation. To elucidate the epigenetic mechanisms regulating physiologic and pathologic FasL transcription, TCR stimulation-responsive promoter histone modifications in normal and alcohol-exposed primary human CD4+ T cells were examined. TCR stimulation of normal and alcohol-exposed cel...

The solvent perturbation behavior and acetylation characteristics of the tyrosine residues in the subtilisins have been examined. The availability of the phenolic side chains both to acetylation and solvent is high, in agreement with the proposed x-ray structure for subtilisin BPN’. Spectroscopic studies on N, 0-diacetyltyrosine, N-acetyltyrosine, and 3-nitrotyrosine in water-dioxane mixtures s...

Abstract Lysine-ε-acetylation (Kac) is a post-translational modification (PTM) that critical for metabolic regulation and cell signaling in mammals. However, its prevalence importance plants remain to be determined. Employing high-resolution tandem mass spectrometry, we analyzed protein lysine acetylation five representative Arabidopsis organs with 2 ~ 3 biological replicates per organ. A total...

Nε-Lysine acetylation has been recognized as a ubiquitous regulatory posttranslational modification that influences a variety of important biological processes in eukaryotic cells. Recently, it has been realized that acetylation is also prevalent in bacteria. Bacteria contain hundreds of acetylated proteins, with functions affecting diverse cellular pathways. Still, little is known about the re...

A cellulose derivative substance called acetate is frequently used in the manufacturing of photographic film, fiber, membranes, and bioplastics. However, availability sources for acetate's raw material does not support this demand. Paper waste has a high content. Therefore, aim research to synthesize from α-cellulose paper. In order separate alpha paper study, delignification process with NaOH ...