The authors have developed a method for large-scale isolation of metaphase chromosomes from HeLa cells. The distinguishing feature of this method is the use of a pH sufficiently low (about 3) to stabilize the chromosomes against mechanical damage. Many milligrams of fairly pure, morphologically intact chromosomes can be isolated in 8 hr or less of total working time. The isolated chromosomes co...

1. Spirochoeta gallinarum can be cultivated in suitable artificial media for many successive generations and probably for indefinite periods. The presence of fresh tissue and a certain amount of oxygen seems to be essential for its growth. No perceptible odor is produced in the cultures. 2. The maximum growth of Spirochoeta gallinarum is reached on about the fifth day, but the phase of degenera...

Volunteer potatoes are difficult to control in onions and can greatly reduce onion growth and yield. Herbicides and cultivation were evaluated for control of simulated volunteer potatoes in onions in 1996 and 2000. Three interrow cultivations did not control potatoes in the onion row and the remaining plants reduced onion yield 50 and 73% compared with the hand-weeded checks. Three applications...

In vitro cultivation of Plasmodium falciparum is critical for studying the biology of this parasite. However, it is likely that different in vitro cultivation conditions influence various aspects of the parasite's life cycle. In the present study two P. falciparum isolates were cultivated using the two most common methods, in which AlbuMAX or human serum as additives are used, and the results w...

Rifamycins are broad spectrum and important antibiotics used in the treatment of pathogenic diseases. They are generally produced by the fermentative cultivation of Amycolatopsis mediterranei, using submerged cultivation system. The medium composition greatly influences the antibiotic production. Trace elements are one of the major factors need to be optimized to enhance the production of rifam...

BACKGROUND Environmental as well as cultivation factors may greatly influence the chemical composition of plants. The main factors affecting the chemical composition of foodstuff are level and type of fertilizer (conventional and organic cultivation systems), location or soil type, and year of harvest. Organic foods are defined as products that are produced under controlled cultivation conditio...

2-bromoethanesulfonate (BES) is a structural analogue of coenzyme M (Co-M) and potent inhibitor of methanogenesis. Several studies confirmed, BES can inhibit CH4 prodcution in rice soil, but the suppressing effectiveness of BES application on CH4 emission under rice cultivation has not been studied. In this pot experiment, different levels of BES (0, 20, 40 and 80 mg kg-1) were applied to study...

Cultivation of wild-harvested plant species has been proposed as a way of reducing over-exploitation of wild populations but lack of technical knowledge is thought to be a barrier preventing people from cultivating a new species. Training programmes are therefore used to increase technical knowledge to encourage people to adopt cultivation. We assessed the impact of a training programme aiming ...