The presence of 10 mM sodium molybdate has a profound effect on the physical behavior of glucocorticoid receptors submitted to ammonium sulfate precipitation or to DEAE-cellulose chromatography. Molybdate inhibits the inactivation of the unoccupied receptor and prevents the transformation of the steroid-bound receptor that occurs when rat liver cytosol is precipitated with ammonium sulfate. The...

Proteoglycans were extracted from bovine cornea with 4M-guanidinium chloride and purified by CsCl-density-gradient centrifugation. Under associative conditions two fractions were found: one capable of forming assemblies of high molecular weight and another lacking this property. The heavier fraction (density 1.59 g/ml) was eluted as a single retarded peak from Sepharose 2B, but on DEAE-Sephadex...

Glucocorticoid-receptor complexes in cytoplasm from normal lymphoid cells incubated with [3H]dexamethasone can be re solved into three different components. Two of these correspond to the well-established activated and nonactivated forms, while the third appears similar to the mero-receptor complex first described by Sherman ef al. (Fed. Proc., 37: 167-173, 1978). Based on their differential af...

Caulerpa lentillifera is a type of green seaweed that cultivated in tropical and subtropical areas. The objectives this study were to determine the characteristics sulfated polysaccharides from C. evaluate its antidiabetic activity. In initial process study, samples macerated with ethanol (1:10). Then, maceration residue was extracted an accumulator at 75 °C for three hours. crude extract yield...