PURPOSE OF REVIEW To summarize the evidence concerning human leukocyte antigen (HLA) epitope mismatch analysis as a means to predict donor-specific antibody (DSA) development and allograft survival. RECENT FINDINGS HLA epitope mismatch analysis outperforms traditional whole molecule antigen mismatch for predicting the risk of de-novo DSA development. By analyzing the number of epitope mismatc...

background: acute myeloid leukemia (aml) with translocation (3,3) is a form of aml that may present de novo or may arise from a previous myelodysplastic syndrome. it is often associated with normal or elevated peripheral blood platelet count and increased bone marrow megakaryocytes with associated multi lineage dysplasia. a subset of patients present with hepatosplenomegaly while a few cases ha...

To clarify the contributions of amidophosphoribosyltransferase (ATase) and its feedback regulation to the rates of purine de novo synthesis, DNA synthesis, protein synthesis, and cell growth, mutated human ATase (mhATase) resistant to feedback inhibition by purine ribonucleotides was engineered by site-directed mutagenesis and expressed in CHO ade (-)A cells (an ATase-deficient cell line of Chi...

Cytosine DNA methylation is an ancient form of epigenetic information found in all kingdoms of eukaryotes. Functions for DNA methylation-mediated gene silencing include imprinting and repression of transposable elements. In mammals, methylation occurs almost exclusively in the CG context. Maintenance of the methyl-mark persists through function of the DNA methyltransferase, DNMT1, whereas the i...

De novo malignancies are one of the current problems in patients with organ transplantation. The incidence has been considered to be higher as a result of increases of oncogenic viruses in immunosuppressed organ recipients. Published reports have shown increased incidence of de novo tumors such as malignant lymphomas and cutaneous neoplasms but decreased incidence of breast cancer. A variety of...

Changes in the locations and boundaries of heterochromatin are critical during development, and de novo assembly of silent chromatin in budding yeast is a well-studied model for how new sites of heterochromatin assemble. De novo assembly cannot occur in the G1 phase of the cell cycle and one to two divisions are needed for complete silent chromatin assembly and transcriptional repression. Mutat...

Recent advancements in computational methods for protein-structure prediction have made it possible to generate the high-quality de novo models required for ab initio phasing of crystallographic diffraction data using molecular replacement. Despite those encouraging achievements in ab initio phasing using de novo models, its success is limited only to those targets for which high-quality de nov...

The flux activities of de novo and salvage purine synthesis were compared in rat hepatoma 3924A cells in various growth phases. The initial rate assays of [I4C]adenine, ("C]hypoxanthine, and |"C]guanine incorporation yielded Michaelis-Menten kinetics with A'msof 5, 7, and 7 #tM,respectively. After replating plateau phase cells in lag and log phases the activity of purine de novo pathway increas...

The de novo silencing of transposable elements in plants and animals is mediated in part by RNA-directed chromatin modification. In flowering plants, AGO4 has been seen as the key argonauteprotein in the RNA-directed DNA methylation pathway that links the plant-specific RNA polymerase V with the de novo DNA methyltransferase DRM2 (Zhong et al,2014). Two recent papers in The EMBO Journal strongl...