A mass spectrometry (MS) method is described here that can reproducibly identify hundreds of peptides across multiple experiments. The method uses intelligent data acquisition to precisely target peptides while simultaneously identifying thousands of other, nontargeted peptides in a single nano-LC-MS/MS experiment. We introduce an online peptide elution order alignment algorithm that targets pe...

BACKGROUND To determine changes in prevalence of parental and childhood asthma in Merseyside between 1991 and 2006. METHODS Four standardized cross-sectional respiratory surveys using a parent-completed questionnaire were completed in 1991 (n = 1171), 1993 (n = 2368) 1998 (n = 1964) and in 2006 (n = 1074) among primary school children attending the same schools in lower socio-economic areas o...

Measurement of steady-state rates of unwinding of double-stranded oligonucleotides by helicases is hampered due to rapid reannealing of the single-stranded DNA products. Including an oligonucleotide in the reaction mixture which can hybridize with one of the single strands can prevent reannealing. However, helicases bind to single-stranded DNA, therefore the additional oligonucleotide can seque...

We investigate the scattering and absorption of light by random ballistic aggregates of spherical monomers. We present general measures for the size, shape, and porosity of an irregular particle. Three different classes of ballistic aggregates are considered, with different degrees of porosity. Scattering and absorption cross sections are calculated, using the discrete dipole approximation (DDA...

In this study, a control strategy to improve the fuel economy was proposed for a PHEV(plug-in hybrid electric vehicle) considering driver tendency. To identify the driver tendency, a driver model which reflects the driver tendency was developed using the driving data. The target PHEV model was developed based on MATLAB/Simulink and validation of the vehicle model was performed by experiments. F...

Helicases are molecular motor enzymes that unwind and translocate nucleic acids. One of the central questions regarding helicase activity is whether the process of coupling ATP hydrolysis to DNA unwinding requires an oligomeric form of the enzyme. We have applied a pre-steady-state kinetics approach to address this question with the bacteriophage T4 Dda helicase. If a helicase can function as a...

Perfusionists should be able to verify diagnosis and recognize the forms of von Wille brand disease (vWD) which may require specific coagulation management during open heart procedures. von Willebrand factor (vWF) plays two distinct and important roles in clot formation subsequent to vascular injury. First, it promotes platelet binding to subendothelial surfaces and other platelets (platelet ag...

The goal for video game AI (artificial intelligence) is to generate AI that is both challengeable and satisfactory. Most existing game AI is implemented by FSM (finite state machine) which has drawbacks in the three respects: requirement of designer’s intensive participation; no existence of meta-programming; no planning and looking forward. Contributions of the paper is to propose CI (computat...

Chitosan is a widely investigated biopolymer in drug and gene delivery, tissue engineering and vaccine development. However, the immune response to chitosan is not clearly understood due to contradicting results in literature regarding its immunoreactivity. Thus, in this study, we analyzed effects of various biochemical properties, namely degree of deacetylation (DDA), viscosity/polymer length ...