BACKGROUND Application of assisted reproductive technologies in buffaloes is limited to some extent by farmers' inability to detect oestrus because of its poor expression. The present study aimed at investigating reliability of a milk progesterone enzyme-linked immunosorbent assay (ELISA) to assess the ovarian cyclicity during post partum, oestrus and post-breeding periods in water buffaloes. ...

Aim: A polyherbal galactogogue based on indigenous knowledge of tribles (Bhagats) belonging to Patalkot (Madhya Pradesh), Dangs (Gujarat) and Aravallis (Rajasthan) was evaluated on lactating Surti buffaloes (medium sized buffalo breed of South Gujarat which is best suitable for small and marginal farmers as well as landless labourers/tribal with less input resources). Materials and Methods: A t...

Incidence S. nasale (causative agent of nasal schistosomiasis) in graded murrah buffaloes was studied. 4(8%) among healthy and 46(92%) among suspected buffaloes were diagnosed with nasal schistosomiasis. Area under investigation was highly irrigated giving ample scope for exposure of animals to cercaria of S. nasale. Buffaloes showing signs of reduced water intake, reduced milk yield, normal bo...

Serum progesterone and estradiol-17β levels were estimated in Nili Ravi buffaloes (n=190) with and without fetal membranes retention in three agro-ecological zones of Punjab. The results showed that serum progesterone concentration was significantly higher (P<0.05) in buffaloes with retention of fetal membranes (RFM) compared to buffaloes without retention of fetal membranes (NRFM). The compari...

This investigation was conducted to identify polymorphism of the leptin gene in Holstein cows, Mazandarani native cattle and buffaloes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The frequencies of A and B alleles were 0.4 and 0. 6 in Holstein cows, 0.56 and 0.44 in Mazandarani native cattle, 0.61 and 0.35 in buffaloes, respectively. The heterozygote genoty...

The present study compares the effect of various lectins; phytohaemagglutinin (PHA), Pokeweed mitogen (PWM) and Concanavalin-A (Con A) on short term lymphocyte culture and morphology of chromosome in cattle and buffaloes. For the comparative studies, the whole blood culture from 5 cattle and 5 buffaloes in triplicate were set up by adding 3 different types of lectins. Results based on physical ...

The rickettsia Anaplasma marginale is the etiologic agent of bovine anaplasmosis, an important tick-borne disease affecting cattle in tropical and subtropical regions of the world. In endemic regions, the genetic diversity of this pathogen is usually related to the high prevalence of the disease in cattle. The major surface protein 1 alpha (MSP1a) has been used as a marker to characterize the g...

A study was undertaken to assess the sensitivity and specificity of Counter Immunoelectrophoresis (CIE) in the diagnosis of hydatidosis in buffaloes using sera samples collected at the time of slaughter. One hundred sera samples from buffaloes were screened for hydatidosis using fertile buffalo hydatid cyst fluid as antigen by CIE. Out of the 100 sera samples used, 61 were positive for hydatid ...

We aimed to determine the seroprevalence of infection with Neospora caninum, Leptospira, and bovine herpesvirus type 1 and risk factors associated with these infections in water buffaloes in Veracruz State, Mexico. Through a cross-sectional study, 144 water buffaloes (Bubalus bubalis) raised in 5 ranches of Veracruz were examined for anti-N. caninum and anti-bovine herpesvirus type 1 antibodies...

Research into the application of electrocardiography in buffaloes has been lacking when compared to other species. However, identification of altered health states, whether for identification of healthy animals or for the measurement of treatment effects is vital for research. Several electrocardiogram (ECG) changes were shown to occur in buffaloes naturally infected with Theileria annulata inc...