OBJECTIVE To describe the morphologic spectrum of metastatic malignant melanoma (MM) cells involving the breast and to explore the diagnostic utility of HMB45, Mart-1, Melan-A and T311 (antityrosinase) antibodies in fine needle aspiration material of MM metastatic to the breast. STUDY DESIGN Cytologic material from 21 cases (18 women) was reviewed for cytomorphology (epithelioid, spindled, mi...

BACKGROUND To evaluate the expression levels of cyclin D1 in breast papillomas and papillary carcinomas, and to analyze the types of cells that co-express cyclin D1 with cytokeratin 5/6 (CK 5/6) or with cytokeratin 8/18(CK 8/18). METHODS Fifty-nine cases of papillary lesions including 36 papillomas and 23 intracystic papillary carcinomas were examined. Cyclin D1, CK 5/6 and CK 8/18 expression...

Monoclonal antibodies were used in indirect immunofluorescence and immunoblot studies to examine the expression of four different classes of intermediate filaments, namely, neurofilaments, glial filaments, cytokeratin, and vimentin, in NTERA-2 cl.D1 (NT2/D1) pluripotent human embryonal carcinoma (EC) cells, and in the neurons derived from these cells by differentiation induced with retinoic aci...

Excessive fibrin deposition in the peritoneum is thought to be involved in the development of encapsulating peritoneal sclerosis (EPS), an important cause of morbidity and mortality in peritoneal dialysis patients. We investigated fibrin-induced epithelial-to-mesenchymal transition (EMT) of peritoneal mesothelial cells (PMCs) as a possible mechanism of fibrin involvement in EPS. In vitro, fibri...

Real-time, single-cell multiplex immunophenotyping of circulating tumor cells (CTCs) is hypothesized to inform diagnosis of tissue of origin in patients with carcinoma of unknown primary (CUP). In 20 to 50% of CUP patients, the primary site remains unidentified, presenting a challenge for clinicians in diagnosis and treatment. We developed a post-CellSearch CTC assay using multiplexed Q-dot or ...

Ovarian metastasis originating from bronchioloalveolar carcinoma (BAC) has not been reported previously. We report a 63-year-old Chinese woman who was diagnosed as BAC with pleural metastasis in 1997. Four years later, she complained of vaginal bleeding, and a pelvic mass was discovered by an abdominal computerized tomography scan. Tumor debulking and total hysterectomy with bilateral salpingo-...

Cytokeratin 1, an intermediate filament keratin, was isolated as a partner of the tyrosine kinase Src from neuroblastoma NMB7 cells. The cytokeratin 1-Src complex was found to be associated with the molecular scaffolder RACK1 (receptor for activated protein kinase C). Interestingly, the cytokeratin 1-Src-RACK1 complex was found to actively bind with membrane receptors such as integrin beta1. We...

stem cells are the cells having some distinguishing characteristics like longevity, high capacity of self-renewal and differentiation, quiescence and highly error-free proliferation. almost all stem cells have the potential of lineage reprogramming, i.e. inter-conversion of cell lineages. they also have the potential to differentiate into almost all kinds of cells. these cells have been found i...

The in situ proximity ligation assay (isPLA) is an increasingly used technology for in situ detection of protein interactions, post-translational modifications, and spatial relationships of antigens in cells and tissues, in general. In order to test its performance we compared isPLA with immunofluorescence microscopy by analyzing protein interactions in cytoplasmic protein aggregates, so-called...

Although several cases of signet-ring cell carcinoma (SRCC) in the colorectum have been reported [1,2], there have been no reports of SRCC in the anus. The case presented here is the first reported case of primary pure SRCC of the anus. A 49-year-old man presented with anal bleeding. Colon endoscopy revealed a polyp in the anus, and polypectomy was performed The polypectomy specimen showed pure...