tissue culture methods provide tools to supplement traditional methods for collection, propagation and preservation of endangered plant species. in this study, in vitro propagation of diaphanoptera khorasanica rech.f., a rare and threatened plant species with limited distribution range and population was investigated. this species has a potential as an ornamental plant. single node explants wer...

Fast freezing and slow thawing Salmonella anatum cells in various milk components inactivated from 20 to 98% of the cells and damaged 40 to 90% of the cells surviving the treatments. Injured cells failed to form colonies on a selective medium (xylose-lysine-peptone agar with 0.2% sodium deoxycholate) but did form colonies on a nonselective plating medium (xylose-lysine-peptone agar). The major ...

Ficus religiosa has great mythological, religious, and medicinal importance in the culture of India and Nepal since times immemorial. The present paper was done to evaluate the potential of sodium hypochlorite in controlling the contamination of F. religiosa micro-propagation and seed germination of F. religiosa. In this study, a factorial experiment based on a completely randomized design with...

Somatic embryogenesis was initiated in C. sativus L. from shoot meristems on LS medium containing BAP (2×10-5 M)+NAA (2×10-5 M). Various stages of somatic embryogenesis were observed in the same medium and the development was asynchronous. Somatic embryo development proceeded through well recognized sequences (globular to embryoids) with clearly discernible bipolar regions. Matured embryos coul...

plant regeneration was achieved in verbascum speciosum schard. via organogenesis and somatic embryogenesis by culture of mature embryo explants. two types of calli, embryogenic and non-embryogenic, were induced from mature embryo explants on murashige and skoog (ms) medium supplemented with different concentrations of benzyl adenine (ba) and α-naphthalene acetic acid (naa). in order to further ...