Microfluidic technology holds great promise for the creation of advanced cell culture models. In this review, we discuss the characterization of cell culture in microfluidic systems, describe important biochemical and physical features of the cell microenvironment, and review studies of microfluidic cell manipulation in the context of these features. Finally, we consider the integration of anal...

The advantages of using electron microscopy for rapid diagnosis of virus infection from clinical specimens, for identification of virus isolates with unusual properties, and for monitoring endogenous agents in cell cultures are illustrated by several actual cases that have occurred over the years. The importance of using morphological characteristics of viruses for initial identification is emp...

A new microtransfer technique is described which uses a modified, V-bottom, disposable, 96-cup plastic plate that permits serial dilutions and direct rapid transfer to a recipient micro cell culture plate.

Studying evolution in the laboratory provides a means of understanding the processes, dynamics and outcomes of adaptive evolution in precisely controlled and readily replicated conditions. The advantages of experimental evolution are maximized when the selection is well defined, which enables linking genotype, phenotype and fitness. One means of maintaining a defined selection is continuous cul...

human conjunctiva cell cultures. Following treatment with mithramycin, RNA synthesis was decreased by 85% in mouse embryo and BHK-21 cell cultures and by 45% in human conjunctiva cell cultures when compared to controls. The inhibition of RNA synthesis in BHK-21 cells was dosedependent and encompassed all cellular types of RNA. Although mithramycin inhibited protein and DNA syntheses in BHK-21 c...

The authors would like to correct Fig 1, as errors were introduced in the preparation of this figure for publication. In Fig 1B, the bld2-6; BLD2 panel and the pf15-1; PF15HA panel are duplicates. The authors regrew the cell cultures in these panels, as well as the parental strains shown in the same panels of Fig 1A, and they have provided a corrected version of Fig 1 here. Copyright: © 2016 Es...