BACKGROUND Previous methods for Kupffer cells (KCs) isolation require sophisticated skills and tedious procedures. Few studies have attempted to explore the self-renewal capacity of KCs in vitro. Therefore, the aim of this study was to establish a simple method for rat KCs isolation and further investigate the mitotic potential of KCs in vitro. METHODS KCs were obtained by performing one-step...

A method of culturing early (13-day) rat embryo neural cells is described. Undifferentiated neural epithelium is disaggregated and cultured in small discrete islands. Cells that destined to differentiate as neurons actively segregate from the other cells in the island and aggregate together into small clumps. Other cells flatten and attach to the substrate and resemble typical fibroblasts throu...

Organotypic slicing of brain tissue from young rodents has been used as a powerful model system for biomedical research [1], [2], [3]. Organotypic slicing complements cell culture and in vivo studies in multiple facets. This system can be useful for investigating manipulation of cellular signaling pathways without the hindrance of the blood-brain barrier while sacrificing fewer animals in the p...

Presterilized mictotiter plates (96 wells) with BHK-21 cells on 5-mm cover slips were successfully used for cell culture isolation of trachoma from 15 infected conjunctival scrapings.

We developed and optimized a simple, efficient and inexpensive method for in vitro culture of peripheral blood lymphocytes from the Brazilian tortoise Chelonoidis carbonaria (Testudinidae), testing various parameters, including culture medium, mitogen concentration, mitotic index, culture volume, incubation time, and mitotic arrest. Peripheral blood samples were obtained from the costal vei...

Conventional 3D culture is typically performed in multi-well plates (e.g. 12 wells). The volumes and dimensions necessitate relatively large numbers of cells and fluid exchange steps are not easily automated limiting throughput. 3D microchannel culture can overcome these challenges simplifying 3D culture processes. However, the adaptation of immunocytochemical endpoint measurements and the vali...

common yew taxus baccata l. is an evergreen, slow growing   tree, indigenous of hyrcanian forests in iran. discovery of paclitaxel as an   anti tumor agent in the bark of taxus has increased importance of this   plant for treatment of breast and ovary cancer, especially 7000 women die annually   due to breast cancer in iran. regarding destructive method of paclitaxel   production and protection...

The clinical application and reliability of the leukocyte culture method for the diagnosis of freemartinism were examined and the length of time that blood samples could be held at room temperature and in the refrigerator prior to culturing, was investigated. The chromosome findings by the leukocyte culture method in 14 freemartins and 9 non-freemartin females belonging to heterosexual twins or...

1950;17;202-205 Dis Chest SCHWARTZ MARTIN M. CUMMINGS, MARGARET C. DRUMMOND and HARRIET B. Sensitivity Testing A Slide Culture Method for Streptomycin http://chestjournal.chestpubs.org/content/17/2/202 can be found online on the World Wide Web at: The online version of this article, along with updated information and services ) ISSN:0096-0217 http://chestjournal.chestpubs.org/site/misc/reprints...