Maintenance of liver-specific function of hepatocytes in culture is still difficult. Improved culture conditions may enhance the cell growth and function of cultured cells. We investigated the effect of three-dimensional culture under flow conditions, and the influence of surface modifications in hepatocyte cultures. Hepatocytes were harvested from Lewis rats. Cells were cultured on three-dimen...

introduction: umbilical cord blood is a rich source of hematopoietic cells and can be used in the clinical setting for hematopoietic cell transplantation in graft versus host disease (gvhd). a number of different drugs have been used as prophylaxis against gvhd including corticosteroids, cyclosporine a (cy a) and fk506. in this study, the in vitro effects of fk506 on cord blood mononuclear cell...

Reasoning with domains containing continuous numeric change remains an open challenge in planning. In this paper, we discuss how an established temporal planner, CRIKEY3, can be adapted to plan in domains containing linear continuous numeric change. In particular, we discuss how an LP can be used to scheudule action choices, and how the LPRPG heuristic (hitherto for non-temporal planning) can b...

Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve. Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The tot...

UNLABELLED A method is described for growth of a Lactobacillus plantarum starter culture in jars of commercially available pasteurized fresh-pack kosher dill cucumbers so that jars can be used to inoculate commercial scale cucumber fermentation tanks. A procedure is also described to transfer lactic acid bacteria from frozen storage in MRS broth into cucumber juice and commercial jars of kosher...

in this paper, a novel method for ranking of fuzzy numbers are proposed. in this method, decision maker is defined based on the center of mass at some cuts of a pair of fuzzy numbers in discreet version and center of mass on all of cuts of a pair of fuzzy numbers in continuous version. our method can rank more than two fuzzy numbers simultaneously. also, some properties of methods are described...

Four hundred and forty-seven human tumor specimens were accessioned and processed for clonogenic assay, yielding 374 specimens, representing 23 different histiotypes, adequate for culture. Different levels of viable cell inoculum density produced contrasting effects between 255 solid tumors as compared to 72 malignant effusions and 47 bladder washings. All parameters for solid tumor growth were...

We compare the numeric performance of C, C# and Java on three small cases.