our aim in this study was to identify and characterize allergic proteins in cooked wolf herring fish. we heated the crude extract alternatively at 50, 60, 70, 80, 90, and 100°c for one hour and results were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). also, proteins were immunoblotted with fish-sensitive patients’ sera. the major allergenic proteins were ide...

We investigated anticancer effects of the crude polysaccharides (CPs) isolated from Ecklonia cava enzymatic extracts using AMG, Viscozyme, Protamex, and Alcalase enzyme against a colon cancer cell line, CT26 cells. Among them, the CP of Protamex extract (PCP) contained the highest fucose and sulfated group contents and showed the highest growth inhibitory effect against CT-26 cells. In addition...

Date palm seed (Phoenix dactylifera) (DPS) extract has been used worldwide in pharmaceutical agent due to its constituents. This study aimed to assess the effects of DPS extract as a dietary supplementation at on the body composition, lipid peroxidation and tissue quality of common carp (Cyprinus carpio) based on the total volatile nitrogen (TVN) test. Common carp juveniles weighing ca 32 g wer...

To date, enzymatic diagnosis of Gaucher’s disease via a fluorometric assay procedure which utilizes 4-methylumbelliferyl-P-D-glucopgranoside as a substrate has not been possible when liver serves as the source of enzyme since currently employed fluorometric procedures cannot adequately differentiate between a broad-specificity fl-glucosidase and lysosomal glucocerebrosidase activities in crude ...

Methyl mercaptan is a naturally occurring compound known to be formed and utilized by living organisms. It can arise in microorganisms (1) and in the rat (2) from methionine. It is rapidly oxidized in the rat, and its methyl carbon atom is incorporated into a number of compounds in this animal (3). In yeast cultures it promotes the accumulation of thiomethyl adenosine (4). Reported here is a re...

Terminalia bellerica Roxb. (Family: Combretaceae) has been valued in Indian system of medicine for treatment of wide range of diseases and reported to have antioxidant properties. In the present study, the free radical scavenging activity and antioxidant potential of acetone extract/fractions of its fruit was investigated using in vitro assays, including scavenging ability against 2,2'-diphenyl...

The antitumor activity of extracts of Centaurea ainetensis (C. ainetensis), a plant endemic to Lebanon, was investigated in human colon carcinoma cells. At concentrations that were non-cytotoxic to normal human intestinal epithelial cells, the crude extract inhibited the proliferation of a host of colon-derived cancer cells. The crude extract effect was then investigated in HCT-116 (p53+/+) cel...

Phenolic compounds were extracted from red lentil seeds using 80% (v/v) aqueous acetone. The crude extract was applied to a Sephadex LH-20 column. Fraction 1, consisting of sugars and low-molecular-weight phenolics, was eluted from the column by ethanol. Fraction 2, consisting of tannins, was obtained using acetone-water (1:1; v/v) as the mobile phase. Phenolic compounds present in the crude ex...

BACKGROUND The issue of resistance in malarial infection makes development of novel drugs a necessity. An alternative source for discovering such drugs is natural products. Croton macrostachyus H. (Euphorbiaceae) is used in Ethiopian folklore medicine for the treatment of malaria and found to possess antimalarial activity in vitro. However, no further scientific investigations have been carried...

Lipases are hydrolases used in various sectors such as the food, pharmaceutical and chemical synthesis industries. In this study, epiphytic microorganisms were isolated from Serra of Ouro Branco State Park (Minas Gerais, Brazil) subsequently evaluated for their ability to produce extracellular lipases. Among 46 strains, 25 presented positive results lipase production agar plate screening assay....