PURPOSE Ripasudil (Glanatec), a selective Rho-associated coiled coil-containing protein kinase (ROCK) inhibitor, was approved in Japan in September 2014 for the treatment of glaucoma and ocular hypertension. The purpose of this study was to investigate the effect of ripasudil eye drops on corneal endothelial morphology, as ROCK signaling is known to modulate the actin cytoskeleton. METHODS Mo...

Corneal thickness is tightly regulated by its boundary endothelial and epithelial layers. The regulated set-point of corneal thickness likely shows inter-individual variations, changes by age, and response to stress. Using anterior segment-optical coherence tomography, we measure murine central corneal thickness and report on body size scaling of murine central corneal thickness during aging. F...

OBJECTIVE/METHOD A 50-year-old hyperopic woman requested information about refractive surgery. Vesicle and band lesions at Descemet's membrane and endothelium were suggestive of posterior polymorphous dystrophy (PPD). Lower than normal endothelial cell densities were detected. A corneal refractive procedure was not recommended in this case. RESULTS/CONCLUSIONS The presence of vesicles and ban...

We investigated the current reformulation of the commercially available intraocular preparation of carbachol for its effect on corneal thickness and endothelial cell ultrastructure. A 15-minute perfusion in the specular microscope with 0.01% carbachol caused a significant increase in the corneal swelling rate, which returned to control values after 75 minutes of reperfusion with Krebs Ringer bi...

PURPOSE To investigate the effects of insulin, epidermal growth factor (EGF), and the corneal storage media--DexSol--at 24 and 48 hours on DNA synthesis in confluent primary cultures of bovine corneal endothelial cells. METHODS Flow cytometry was used to measure changes in DNA synthesis. This technique allows a large number of cells to be counted and sorted into G1, S, and G2/M phases of the ...

Fuchs endothelial corneal dystrophy (FECD) is the most common corneal dystrophy and frequently results in vision loss. Hallmarks of the disease include loss of corneal endothelial cells and formation of excrescences of Descemet's membrane. Later stages involve all layers of the cornea. Impairment of endothelial barrier and pump function and cell death from oxidative and unfolded protein stress ...

'Snailtracks' (grey-white streaks and patches on the posterior corneal surface which occur postoperatively) have recently been implicated as possible markers of corneal endothelial cell damage. We have examined this phenomenon in vivo with the slit-lamp and specular microscope. We recognised three types of linear form and, in contradiction of other observers, found that each was always coincide...

Purpose: To compare central corneal thickness (CCT), corneal endothelial cell density, and lens capsule thickness in normotensive patients with and without pseudoexfoliation syndrome (PXS) Methods: This was a prospective, comparative, descriptive study. Normotensive candidates for cataract surgery with (study group) and without (control group) PXS were enrolled in the study. CCT and corneal end...

Three genetic corneal dystrophies [congenital hereditary endothelial dystrophy type 2 (CHED2), Harboyan syndrome and Fuchs endothelial corneal dystrophy] arise from mutations of the SLC4a11 gene, which cause blindness from fluid accumulation in the corneal stroma. Selective transmembrane water conductance controls cell size, renal fluid reabsorption and cell division. All known water-channellin...

PURPOSE The aim of this study was to quantify epithelial, stromal, and endothelial cell density, and subbasal nerve morphology in young patients with type 1 diabetes mellitus with and without diabetic retinopathy. METHODS A total of 28 young patients (mean age, 22.86 ± 9.05 years) with type 1 diabetes, with (n = 18) and without (n = 10) retinopathy, and 17 age-matched healthy control subjects...