Purpose. To study the corneal nerve morphology and its importance in unilateral keratoconus. Materials and Methods. In this prospective cross-sectional study, 33 eyes of 33 patients with keratoconus in one eye (Group 3) were compared with the other normal eye of the same patients (Group 2) and 30 eyes of healthy patients (Group 1). All patients underwent detailed ophthalmic examination followed...

PURPOSE To report corneal stem cell allografts in a patient with a persistent epithelial defect as well as corneal melting and perforation due to severe ultraviolet light burn and thermokeratoplasty treatment for keratoconus. METHODS A 21-year-old female patient with corneal melting, perforation and a persistent epithelial defect in her left eye secondary to iatrogenic treatment for keratocon...

PURPOSE To characterize the extracellular matrix (ECM) formed by corneal stromal cells after injection into the vitreous. This will provide a basis for future studies on the function of corneal ECM macromolecules. METHODS Cell line from rabbit dermal fibroblasts (RAB9) and primary cultures of rabbit corneal stroma fibroblasts (NRCF) were grown to confluence. For each cell type, approximately ...

PURPOSE To investigate the effect and safety of a selective Rho kinase inhibitor, ripasudil 0.4% eye drops, on corneal endothelial cells of healthy subjects. DESIGN Prospective, interventional case series. METHODS In this study, 6 healthy subjects were administered ripasudil 0.4% in the right eye twice daily for 1 week. Morphological changes and corneal endothelial cell density were examine...

baradaran rafii ar, md; motevasseli t, md*; yazdizadeh f, md; ansari sh, md; karimian f, md; sadoughi mm, md; kiani h ocular tissue engineering research center, shahid beheshti university of medical sciences, tehran, iran * corresponding author: [email protected] purpose: to compare anterior segment indices measured by two scheimpflug camera; galilei and pentacam. methods: in this observa...

The corneal stromal lesions of a case of lattice dystrophy that fulfilled all the clinical and histopathologic criteria of this disease were examined by light and electron microscopy. The lesions were found to consist of two distinct parts: (1) a nonbirefringent mass of disoriented filaments of approximately one-third the diameter of normal stronml collagen fibrils and (2) a second, birefringen...

PURPOSE To examine the effect of topical ranibizumab on clinically stable corneal neovascularization (NV). METHODS This was a prospective, open-label, monocentric, uncontrolled noncomparative study. Ten eyes of 9 patients with corneal NV received topical ranibizumab (1%) 4 times a day for 3 weeks with a follow-up period of 16 weeks. The main corneal NV outcome measures were: neovascular area,...

PURPOSE We examined histopathologically the anterior ocular segment including the cornea and lens of an eye which had been enucleated in a patient with Peters' anomaly because of untreatable corneal perforation. Special effort was made to differentiate the corneal stromal and endothelial cells, and the stromal extracellular matrix. METHODS Light microscopy, with hematoxylin and eosin staining...

Purpose To evaluate morselized amniotic membrane and umbilical cord (AMUC) eye drops in promoting corneal re-epithelialization. Methods Following a 2-mm diameter central epithelial wound in one eye of 48 normal C57BL/6 mouse corneas, 10 μL of saline with (n = 24) or without (n = 24) AMUC was applied three times a day for 6 days. The corneal epithelial defect was measured using 0.1% fluorescei...

PURPOSE To measure the anterior corneal asphericity (Q) with different corneal diameters. SETTING Department of Physics (Optometry), University of Minho, Braga, Portugal. METHODS Thirty-six eyes of 36 patients were evaluated using a videokeratoscope, and the Q-values were recorded. Topographic data were also analyzed using Vol-CT 6.89 software (Sarver & Associates, Inc) to obtain the Q-valu...