Copper is an essential cofactor for many enzymes, and at over a threshold level, it is toxic for all organisms. To understand the mechanisms underlying copper homeostasis of the gram-positive bacterium Bacillus subtilis, we have performed microarray studies under copper-limiting conditions. These studies revealed that the ycnJ gene encodes a protein that plays an important role in copper metabo...

Copper transporter 1 (CTR1) is a transmembrane protein that imports copper(i) into yeast and mammalian cells. Surprisingly, the protein also mediates the uptake of platinum anticancer drugs, e.g. cisplatin and carboplatin. To study the effects of several metal-binding residues/motifs of hCTR1 on the transport of both Cu(+) and cisplatin, we have constructed Hela cells that stably express a seri...

Assuming the maximum copper level 210 g per cent was administered to the patient, the copper dose administered would be approximately 3.15 mg/day, normal copper intake as quoted by the authors 2-3 mg/day.2 The authors suggest that a dose of six times this amount (20mg) is acceptable. If one takes into account that the average expired copper level was 134 g percent, the actual dose is likely to ...

OBJECTIVE To study the serum zinc, selenium, and copper of priest subject who attended the outpatient Department, Priest Hospital Bangkok for a physical check-up, between March and October 2003 MATERIAL AND METHOD The serum zinc, selenium, and copper of 112 priest subjects, 33 healthy and 79 diseased, compared with a control group of 90 males and 119females Thai volunteers. RESULTS Serum co...

Copper deficiency was induced in eight male buffalo calves by adding molybdenum (30 ppm wet basis) to their diet. Copper status was monitored from the liver copper concentration and a level below 30 ppm (DM basis) was considered as deficient. Haemoglobin, haematocrit, total and differential leucocyte numbers were determined. The functions of peripheral neutrophils were assessed by in vitro phag...

BACKGROUND Inhibitors of hyaluronidase are potent agents that maintain hyaluronic acid homeostasis and may serve as anti-aging, anti-inflammatory, and anti-microbial agents. Sodium copper chlorophyllin complex is being used therapeutically as a component in anti-aging cosmeceuticals, and has been shown to have anti-hyaluronidase activity. In this study we evaluated various commercial lots of so...

Bacterial and viral contamination of touch surfaces allows for transmission of pathogens leading to increased risk of infection. Previous work has demonstrated the antimicrobial properties of copper for contact-killing of microbes for use in hospitals. Less research exists on copper as an antiviral surface and on the effects of nanomaterial copper surfaces in the contact-killing of viruses. Nan...

The unicellular green alga Chlamydomonas reinhardtii is a valuable model for studying metal metabolism in a photosynthetic background. A search of the Chlamydomonas expressed sequence tag database led to the identification of several components that form a copper-dependent iron assimilation pathway related to the high-affinity iron uptake pathway defined originally for Saccharomyces cerevisiae....

Copper levels are elevated in a variety of liver fibrosis conditions. Lowering copper to a certain level protects against fibrosis. However, whether severe copper deficiency is protective against liver fibrosis is not known. The purpose of the present study is to evaluate this question by inducing severe copper deficiency using the copper chelator, tetrathiomolybdate (TM), in a bile duct ligati...