Received 9/25/2003; revised 12/5/2003; accepted 12/9/2003. For correspondence or reprints contact: Wen-Tao Huang, Ph.D., Department of Radiological Technology, Yuanpei University of Science and Technology, 306 Yuanpei Street, Hsinchu 300, Taiwan, ROC. Tel: (886)3-5381183 ext. 8312, Fax: (886)3-5385353, E-mail: [email protected] A series of tetradentate N2O2 Schiff-base compounds were syn...

Glutaraldehyde-fixed membranes from rabbit kidney cortex were used to characterize binding of monomeric adenosine deaminase to the adenosine deaminase complexing protein. With the use of bovine adenosine deaminase it was shown that enzyme binding is a saturable, high affinity process. The K value for binding of the bovine enzyme was 11 nM. Maximum enzyme binding and rate of binding to a constan...

It has been suggested that the complexing type of inactivation in which the inactivator binds reversibly with the enzyme before inactivation cannot be differentiated kinetically from that a slow enzyme conformation change is involved as a first step [Rakitzis (1986) J. Theor. Biol. 122, 247-249]. The kinetics of the substrate reaction during modification of enzyme activity previously described ...