Although Bicinchoninic acid (BCA) has been widely used to determine protein concentration, the mechanism of interaction between protein, copper ion and BCA in this assay is still not well known. Using the Micro BCA protein assay kit (Pierce Company), we measured the absorbance at 562 nm of BSA solutions with different concentrations of protein, and also varied the BCA concentration. When the co...

BACKGROUND RAS protein interactions have predominantly been studied in the context of the RAF and PI3kinase oncogenic pathways. Structural modeling and X-ray crystallography have demonstrated that RAS isoforms bind to canonical downstream effector proteins in these pathways using the highly conserved switch I and II regions. Other non-canonical RAS protein interactions have been experimentally ...

An important milestone in revealing cells' functions is to build a comprehensive understanding of transcriptional regulation processes. These processes are largely regulated by transcription factors (TFs) binding to DNA sites. Several TF binding site (TFBS) prediction methods have been developed, but they usually model binding of a single TF at a time albeit few methods for predicting binding o...

The interactions between lysozyme-imprinted hydrogel and their template protein were studied using adsorption measurements, competitive adsorption experiments, and isothermal titration calorimetry (ITC). The results were compared to the interactions between the imprinted polymer and a reference protein, cytochrome c. Experimental adsorption isotherms and competitive adsorption studies detected ...

Molecules that can control protein-protein interactions (PPIs) have recently drawn attention as new drug pipeline compounds. Here, we report a technique to screen desirable affinity-altered (affinity-enhanced and affinity-attenuated) protein variants. We previously constructed a screening system based on a target protein fused to a mutated G-protein γ subunit (Gγcyto) lacking membrane localizat...

We study an online model of fair division designed to capture features of a real world charity problem. We consider two simple mechanisms for this model in which agents simply declare what items they like. We analyse axiomatic properties of these mechanisms such as strategy-proofness and envy freeness. Finally, we perform a competitive analysis and compute the price of anarchy.

In this paper we investigate parallel searching on m concurrent rays. We assume that a target t is located somewhere on one of the rays and that a group of m point robots has to reach t. Furthermore, we assume that the robots have no way of communicating over distance. Given a strategy S we are interested in the competitive ratio which is deened as the ratio of the time needed by the robots usi...

This paper studies competitive equilibria in economies characterized by the presence of asymmetric information, where non-exclusive contracts are traded on competitive markets and agents may be privately informed over their payoff. For such economies competitive equilibria may not exist when contracts trade at linear prices. We show that (non-trivial) competitive equilibria exist, under general...

One aim of laboratory investigation in neonatal jaundice is to prevent kernicterus from occurring. Estimation of the bilirubin-binding capacity of serum is an important measurement along with total bilirubin determination. With a convenient Sephadex competitive binding procedure, KERNLUTE provides information on whether or not the neonate's bilirubin-binding capacity has been exceeded. Further ...

International donors, long-standing supporters of decentralization reforms in developing countries, often face the challenge of aligning program assistance to the great variety of country governance settings in which many operate. This paper presents a framework for assessing the implications of governance and institutional context for a range of programming challenges, with particular referenc...