Background: Occult bacterial infection is a proposed etiology of low back pain (LBP). However, causative link between LBP and bacteria remains unconfirmed. Herein, we determined the incidence occult discitis in patients receiving surgery for LDH. Methods: Study Design: prospective cohort study. Inclusion criteria: consecutive adult undergoing discectomy symptomatic Exclusion prior epidural ster...

Pfu DNA Polymerase(a)-generated blunt-end PCR fragments can be ligated into the pGEM®-T and pGEM®-T Easy Vectors(b,c) if the fragments are first A-tailed using Taq DNA Polymerase(a). We describe a method for A-tailing these PCR fragments and demonstrate its utility by cloning two different Pfu DNA Polymerase-generated fragments into the pGEM®-T Easy Vector, using the new 2X Rapid Ligation Buffe...

background molecular farming is the production of important recombinant proteins in transgenic organisms on an agricultural scale. interferons are proteins with antiviral and antitumor activities and can be used for viral infections and cancers treatments. objectives this study reports the transformation of inf α2b gene in tobacco plant for the first time in iran. materials and methods interfer...

p73 is a member of the p53 tumor suppressor protein family and induces apoptosis in tumor cells that lack functional p53. It has been demonstrated that methylation of CpG islands in the promoter and exon 1 region may result in silencing of the p73 gene. The aim of this study was to investigate the correlation between p73 gene expression and chemosensitivity in non-small cell lung cancer (NSCLC)...

BACKGROUND Streptococcus pneumoniae is a common etiologic agent of invasive respiratory infections among children under 5 years of age and older adults. Isolation rates of S. pneumoniae by traditional culture techniques are low. AIM To study the sensitivity and specificity of two different DNA extraction methods to amplify the ply gene, applied to three different types of blood culture broths...

The aim of this study was to investigate the expansion capacity and origin of bone marrow-derived mesenchymal stem cells (MSCs) in 34 patients who received a sex-mismatched stem cell transplant (SCT). Polymerase chain reaction (PCR) analysis of the amelogenin gene (AMEL) was used to detect donor-derived MSCs. Cultured MSCs were hybridized with fluorescence in situ hybridization (FISH) probes fo...

the aim of present study was to investigate the effect of individual and co-inoculation of trichoderma species and arbuscular mycorrhizal fungi (amf) on growth, mycorrhization, population of trichoderma, and wilt disease severity in pigeon pea (cajanus cajan l millsp). three species of trichoderma, namely, t. harzianum (th), t. virens (tr), and t. viride (tv) and consortium of amf (myc; mixture...

STATEMENT OF FINDINGS: We developed a real-time detection (RTD) polymerase chain reaction (PCR) with rapid thermal cycling to detect and quantify Pseudomonas aeruginosa in wound biopsy samples. This method produced a linear quantitative detection range of 7 logs, with a lower detection limit of 103 colony-forming units (CFU)/g tissue or a few copies per reaction. The time from sample collection...

We investigated the presence of cagA-like gene of Helicobacter pylori in environmental isolates of Aeromonas spp. from different water samples of Calcutta, India, by colony hybridization using a cagA-specific DNA probe and by PCR with cagA-specific primers. Nucleotide sequencing of five PCR products revealed 97 to 98% homology to canonical cagA of H. pylori 26695 as well as to four clinical H. ...