Erythroid colony formation in methyl cellulose has been used for the measurement of erythropoietin in plasma. Livers from newborn mice less than 24 hr old were found to provide convenient target cells. Newborn mouse liver contains a substantial number of erythroid colony-forming cells (CFU-e) that have a high sensitivity to erythropoietin, the dose--response curve for erythropoietin reaching a ...

OBJECTIVES CD34+ cells and colony forming unit-granulocyte and macrophage (CFU-GM) from human bone marrow were used to investigate the role of Fas/FasL system in the regulation of myelopoiesis. METHODS Fas and FasL expression in CD34+ cells and in day 14 CFU-GM were measured by RT-PCR and immunofluorescence respectively. The functional assays for the CFU-GM were measured by a standard colony ...

A method is described for the partial purification of a granulomonopoiesis inhibitor derived from 200 l-batches equivalent to 1 kg or 2 X 10(12) leukocytes of porcine blood. Serum-free conditioned media were concentrated and separated via ammonium sulfate precipitation, acetone partitioning, ultrafiltration and gel chromatography. The active substance revealed a molecular mass of 500-700 Da and...

G-protein coupled estrogen receptor (GPER) is a transmembrane that mediates non-genomic effects of estrogen. This study aimed to investigate the role GPER in stemness formation and malignancies triple negative breast cancer (TNBC) cells. Spheroids MDA-MB-468 cells were induced by mammosphere culture, proportion CD44+ /CD24−/low stem cell subpopulation was detected. Malignant characteristics, ex...

BACKGROUND The number of CD34+ cells in a peripheral blood stem cell collection is the key factor in predicting successful treatment of hematologic malignancies. Korean Red Ginseng (KRG) (Panax ginseng C.A. Meyer) is the most popular medicinal herb in Korea. The objective of this study was to determine the effect of KRG on hematopoietic colony formation. METHODS Bone marrow (BM) samples were ...

Erythroid colony formation in methyl cellulose has been used for the measurement of erythropoietin in plasma. Livers from newborn mice less than 24 hr old were found to provide convenient target cells. Newborn mouse liver contains a substantial number of erythroid colony-forming cells (CFU-e) that have a high sensitivity to erythropoietin. the dose-response curve for erythropoietin reaching a p...

The role of prolactin (PRL) in supporting the growth of human breast cancer is still unclear. The ability to grow primary breast cancer specimens in the soft agar clonogenic assay in the absence of serum gave us the opportunity to evaluate the growth-promoting effect of PRL and to compare it to that of estradiol in the same tumor samples. PRL was tested both at physiological concentrations (20 ...

Circulating blast cells from the peripheral blood of acute myeloblastic leukemia patients include a subpopulation capable of colony formation in the presence of phytohemagglutinin-stimulated leukocyte-conditioned medium (PHA-LCM). We describe the complete replacement in the blast assay of PHA-LCM by conditioned medium from a human bladder carcinoma cell line, HTB9. Both conditioned media contai...

The aim of the present study was to investigate whether pristimerin affects the bone metastasis, stem cell characteristics and epithelial-mesenchymal transition (EMT) of prostate cancer (PCa) PC-3 cells subjected to hypoxia. The PC-3 cells were cultured under hypoxia or normoxia for 48 h and were then treated with increasing concentrations of pristimerin from 0 to 0.8 µmol/l, under normoxia. Hy...

The appropriate dosage of edaravone—a radioprotective agent—and its effect on tumors are unknown. This study evaluated the effects edaravone intestinal injuries and in mice induced by whole body X-ray irradiation. Small mucositis was C3H/HeNSlc using a single dose (15 Gy). Edaravone (15, 30, 100 mg/kg) administered 30 min before irradiation to evaluate protective effect. After 3.5 days, jejunum...