MicroRNAs regulate self renewal and differentiation of cancer stem cells. There, we sought to identify the expression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study. MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting. After miR-181b was overexpressed in U87 glioma s...

Colon cancer constitutes a major challenge all over the world, which has fourth highest incidence and third mortality rate of cancers. Although therapeutic modalities have been improved in recent years, 5 y survival remains low. Oxymatrine is one main active compounds extracted from roots Sophora flavescens was found with effect for colon Recently, microRNAs recognized as type diagnostic progno...

We evaluated alamarBlue as a metabolic indicator in a standardized assay for the measurement of serum bactericidal activity (SBA) to Haemophilus influenzae type b (Hib) using sera containing natural and vaccine-induced anticapsular (polyribosylribitol phosphate) antibodies. SBA assays with a colorimetric and a fluorometric end point in the presence of alamarBlue were developed and compared to a...

background: yeast infections are increasing cause of morbidity and mortality in immunocompromised patients. in order to perform a dna-based diagnostic test, availability of a rapid and easy-to-perform dna extraction protocol is essential. in the present study we evaluated colony-pcr as the easiest way to amplification of target dna. methods: instead of using templates of purified genomic dna, w...

OBJECTIVE To investigate the relationship between hypoxia and in vitro "stemness" of cancer stem cells (CSCs). METHODS U87 cells, U251 cells and primary glioma cells (n=3) experienced hypoxia. Transmission electron microscopy was done to detect the ultrastructure of these cancer cells; MTT assay to detect the cell growth; flow cytometry to detect cell cycle and CD133 expression; Transwell cha...

Background: This study aimed to investigate the possible involvement of sonic hedgehog (Shh) signaling in the radioresistance of human osteosarcoma cells and the inhibitory effects of emodin on radioresistance. Material/Methods: Human osteosarcoma (OS) cell line MG63 was employed as parent cells. After 30-repeat low-dose (2 Gy) x-ray irradiation on MG63 cells, radioresistant OS cell MG63R cells...

Aim Mycoplasma pulmonis (MP) remains potentially important rodent pathogen causing murine respiratory mycoplasmosis (MRM) which may go undiagnosed due to its asymptomatic nature. In the present study, we carried out clinical, pathological, and molecular investigations of MP-induced MRM in a rat colony. Materials and Methods Two female Wistar rats were observed to be diseased in animal facilit...

Correlation between hyaluronidase (Hyl) activity and biofilm detachment in a few bacterial species was found. However, it is unclear if this association applies to or for more general characteristics. This study determined the production Hyl among isolates from oral cavity of healthy subjects, its with Gram staining group, colony surface morphology bacteria shape. The swab taken tongue, cheek e...

The clonogenic (or colony forming) assay has been established for more than 50 years; the original paper describing the technique was published in 1956. Apart from documenting the method, the initial landmark study generated the first radiation-dose response curve for X-ray irradiated mammalian (HeLa) cells in culture. Basically, the clonogenic assay enables an assessment of the differences in ...

Cisplatin represents an important strategy for gastric cancer treatment. However, cisplatin resistance often leads to failure of therapy as well local reoccurrence. Recently, long non-coding ribonucleic acid was demonstrated exert roles in cancer. Expression measured by real time polymerase chain reaction assay cells from different groups. Cell apoptosis checked with Hoechst methods and cell co...