The matrix metalloproteinases play an important role in matrix degradation, but there is limited information about this family of enzymes in either normal or diseased human liver. In this study, we have examined the synthesis of a 72 kDa type IV collagenase/gelatinase by human hepatic lipocytes in primary culture. Hepatic lipocytes were isolated from wedges of normal human donor liver by Pronas...

An assay system for estimating quantitatively collagenase-like activity present in bone cells has been developed as part of a more general investigation of mechanisms of bone resorption. Methods are described for preparing from bone a C(14)-labeled collagen which is relatively pure and highly resistant to degradation by trypsin although readily broken down by bacterial collagenase. Collagenolyt...

BACKGROUND Ultraviolet radiation from sunlight induces overproduction of reactive oxygen species (ROS) resulting in skin photoaging and hyperpigmentation disorders. Novel whitening and anti-wrinkle compounds from natural products have recently become of increasing interest. The purpose of this study was to find products that reduce ROS in 14 Thai plant extracts. METHODS To determine total phe...

The biosynthesis of human skin collagenase was studied in fibroblast cultures incubated with 13H]leutine under serum-free conditions. The radioactively labeled enzyme was isolated and quantitated directly from culture medium using a highly specific immunoprecipitation method. Sodium dodecyl sulfate gel electrophoresis of the immunoprecipitates showed two radioactive bands tihich co-migrated wit...

p53, a tumor suppressor and a transcription factor, has been shown to transcriptionally activate the expression of a number of important genes involved in the regulation of cell growth, DNA damage, angiogenesis, and apoptosis. In a computer search for other potential p53 target genes, we identified a perfect p53 binding site in the promoter of the human type IV collagenase (also called 72-kDa g...

Platelet-derived growth factor (PDGF) is a mitogen associated with tissue repair, a process involving collagen synthesis and remodelling by interstitial collagenase. This study examines and compares the regulation of interstitial collagenase and collagen gene expression by PDGF-AA and -BB in human fibroblasts. Time-course analysis showed that neither PDGF-AA or -BB had a consistent effect on th...

The urokinase-type plasminogen activator (uPA) and the matrix-degrading metalloproteinases MMP-2 and MMP-9 (type IV collagenases/gelatinases) have been implicated in a variety of invasive processes, including tumor invasion, metastasis and angiogenesis. MMP-2 and MMP-9 are secreted in the form of inactive zymogens that are activated extracellularly, a fundamental process for the control of thei...

Background: Clausena harmandiana (Song Fa) leaves are a source of essential oil, in which the dominant compound is trans-anethole and reported high antioxidant activity but inhibition biological enzymes related to anti-wrinkle limit. Objectives: The objectives this study investigate ability Song Fa leaf oil (SFEO) inhibit that cause skin wrinkles then develop bioactive ingredient nano-drug deli...

This study was designed to investigate the collagen metabolism and tyrosinase activity of Olibanum extracts (OE). The currently known effects of OE are to move blood, calm pain, solve oedema, and promote tissue regeneration. The effect of OE on type I procollagen production and collagenase (matrix metalloproteinase-1, henceforth referred as MMP-1) activity in human normal fibroblasts Hs68 after...

The family of matrix metalloproteinases is a family of closely related enzymes that play an important role in physiological and pathological processes of matrix degradation. The most distinctive characteristic of interstitial collagenases (fibroblast and neutrophil collagenases) is their ability to cleave interstitial collagens at a single peptide bond; however, the precise region of the enzyme...