Eukaryotes, including the unicellular eukaryotes such as yeasts, employ multiple levels of gene regulation. Regulation of chromatin structure through chromatin compaction cascades, and influenced by transcriptional insulators, might play a role in the coordinated regulation of genes situated at adjacent loci and expressed as a co-regulated cluster. Subtelomeric gene silencing, which has previou...

A kinetic model for ethanol fermentation of lactose using yeast Kluyveromyces marxianus DSMZ 5422 is proposed. The model consists of a set of differential equations which account for substrate consumption, ethanol production and biomass production. In the model, it is assumed that alcoholic fermentation is inhibited by ethanol itself and that a different metabolic pathway is set at certain etha...

The Kluyveromyces marxianus yeast recently has gained considerable attention due to its applicability in high-value-added product manufacturing. In order intensify the biosynthesis rate of a target product, reaching high biomass concentrations reaction medium is mandatory. Fed-batch processes are an attractive and efficient way how achieve cell densities. However, depending on physiology partic...

Nowadays electroporation is well established as a method for increasing the permeability of biological membranes aiming to include some kind of molecule inside cells (anticancer drugs, for example) or to extract molecules from cells (enzymes, DNA, etc.). The permeabilized cells show a distribution of hydrophilic pores with diameters of several nanometers in the regions where the induced transme...

This study aims to identify yeasts from spontaneously fermented herbs used for Van herby cheese and evaluate their enzymatic activities. Sequencing of partial 26S rRNA gene revealed the species Pichia membranifaciens (n = 8), Kazachstania servazzii 6), Debaryomyces hansenii 2), Kluyveromyces marxianus fermentans 2). Enzyme profiles were determined using API-ZYM strips. The isolates had diverse ...