This study was aimed at demonstrating associations between peripheral biochemical parameters, endometrial leukocyte esterase (LE) and myeloperoxidase (MPO), and bacterial detection with the degree of endometrial inflammation, and determining the best time postpartum for diagnosing endometritis to predict subsequent fertility in dairy cows. Plasma albumin, blood urea nitrogen (BUN), total choles...

milk samples were collected from quarters of 35 cows with subclinical mastitis (california mastitis test [cmt] positive and somatic cell counts [scc] >500,000 cells/ml in individual quarter foremilk), as well as from 37 healthy controls. compared to the levels observed in milk from healthy quarters, milk from quarters with subclinical mastitis showed elevated sodium (91.97 vs 52.93 mg/dl), chlo...

The aim of the present study was to examine the fertilization ability and mitochondrial function of oocytes derived from cows with or without liver damage. Oocytes were collected from the ovaries of cows with damaged livers (DL) and those of cows with healthy livers (HL), subjected to in vitro maturation, and fertilized in vitro. A significantly high abnormal fertilization rate was observed for...

The article presents the results of a study influence negative energy balance in cows transition period development postpartum uterine pathologies. On sonogram, metritis is presented as hypoechoic uniform thickening layers wall. With pyometra, thinning wall occurs. sonograms obtained during study, cavities considerable diameter (average 67.8 ± 4.6 mm) with content, presence hyperechoic inhomoge...

BACKGROUND Haptoglobin and serum amyloid A are major acute phase proteins in cattle. Dairy cattle often develop pathologic conditions in the peripartum period; acute phase proteins may be useful in their diagnosis. OBJECTIVES The purpose of this study was to compare the accuracy of serum haptoglobin (Hp) and serum amyloid A (SAA) concentrations with clinical health status for diagnosing disea...

STOJEVIĆ, Z., J. PIRŠLJIN, S. MILINKOVIĆ-TUR, M. ZDELAR-TUK, B. BEER LJUBIĆ: Activities of AST, ALT and GGT in clinically healthy dairy cows during lactation and in the dry period. Vet. arhiv 75, 67-73, 2005. ABSTRACT This study examined the activities of aspartate aminotransferase (AST; EC 2.6.1.1), alanine aminotransferase (ALT; EC 2.6.1.2) and gamma-glutamyltransferase (GGT; EC 2.3.2.2) in t...

AIM The aim of this study is to determine serum ceruloplasmin levels in cows with endometritis of varying degrees of severity and to establish whether or not there is a correlation between acute phase protein (APP) levels and biochemical parameters. MATERIAL AND METHODS The study was conducted with 100 Brown Swiss cows (3-8 years of age) on days 28-32 postpartum. Cows were divided into endome...

The overall objective of this study was to evaluate agreement between rectal (RT) and vaginal temperature (VT) measured with the same temperature loggers in dairy cows. Three experiments were conducted. The study began with a validation in vitro of 24 temperature loggers comparing them to a calibrated liquid-in-glass thermometer as a reference method. The association and agreement between the 2...

BACKGROUND The natural incidence of left displacement of abomasum (LDA) in dairy cows was high. The diagnosis of LDA usually relies on characteristic physical exam findings but that transabdominal ultrasound is a useful technique that has been applied to the diagnosis of gastrointestinal diseases of dairy cows in equivocal cases. METHODS Forty dairy cows with LDA were clinically and ultrasono...

Real time-qPCR is the most reliable method for evaluation of mRNA expression levels. However, to obtain accurate results, selection of suitable reference genes is necessary for normalizing the real-time qPCR data. The aim of this research was to validate the expression stability of three potential reference genes (ACTB, GAPDH and UXT) in milk somatic cells of Holstein dairy cattle under differe...