OBJECTIVE To identify the frequency and maternal and neonatal factors associated with meconium-stained amniotic fluid at birth. METHODS Cross-sectional study carried out with 2,441 births at an in-hospital birth center in the city of São Paulo (Southeastern Brazil) in March and April, 2005. The association between meconium-stained amniotic fluid and the independent variables (maternal age, pa...

We reviewed the background information and previous clinical studies that considered the use of allogeneic amniotic tissue and fluid (granulized amniotic membrane and amniotic fluid) in the treatment of chronic diabetic foot wounds. This innovation represents a relatively new approach to wound management by delivering a unique allograft of live human cells in a nonimmunogenic structural tissue ...

The total phospholipid content of the supernatant fluid decreases rapidly with increasing relative centrifugal force when amniotic fluid is centrifuged. Possible explanations for this are discussed. The effect of storage on the total phospholipid content of amniotic fluid at different temperatures and the influence of contamination with blood and meconium are also described.

The changes in concentration of oestrone, oestradiol (-17alpha and -17beta), oestrone sulphate and the oestradiol sulphates have been measured in allantoic and amniotic fluids and in maternal peripheral plasma throughout gestation. Oestrone sulphate was the major oestrone present in all of the fluids. It was measurable in allantoic fluid before Day 52 and reached a peak concentration of 475 ng/...

Literature concerning procoagulant activity of the amniotic fluid and pathomechanism of amniotic fluid embolism (AFE) was surveyed and a new concept of its pathogenesis, called the integrated concept of AFE, was presented. According to this concept, two components of the amniotic fluid are involved: 1) apoptosis-affected amniotic cells showing a special role in the initiation of disseminated in...

Objectives: To confirm three point of care commercial tests for rupture of membranes consistently and accurately detect amniotic fluids from pregnant women, and they are practical and easy to use. Methods: Samples of fresh amniotic fluid were collected in a syringe taken at time of Csection and run on kits (ActimTMPROM, AmniSure®, and ROM +plus®) from three commercially available point of care ...

background premature rupture of membranes (prom) is one of the most common complications of pregnancy that has a major impact on pregnancy outcomes. a diagnostic tool that is non-invasive, specific and quick is needed to predict prom. the purpose of this study was to evaluate the diagnostic power of the vaginal washing fluid prolactin for the diagnosis of premature rupture of membranes and to d...

This article discusses possible applications of cells derived from human amniotic fluid in regenerative medicine, specifically in stroke therapy. Recent studies have evaluated amniotic fluid as a viable source for mesenchymal stem cells in the expansion of cell-based transplantation. Laboratory data have demonstrated the ability of amniotic fluid stem cells (AFSC) to act as biobridges or subdur...

OBJECTIVE To determine the association between the presence of oligohydramnios, determined as an amniotic fluid index ≤ 5 cm and the intra-amniotic inflammatory response, fetal inflammatory response and neonatal outcomes in actively managed preterm prelabor rupture of membranes (PPROM). METHODS Women with singleton pregnancies complicated by PPROM at a gestational age of between 24+0 and 36+6...

Thromb Haemost 2009; 101: 795–796 The role of amniotic fluid cells in the initiation of intra vascular coagulation in amniotic fluid embolism is the topic of the article by Zhou et al. (1) in the current issue of Thrombosis and Haemostasis. This is new information which can be considered as an argument for the basic mechanism of amniotic embolism related to disseminated intravascular coagulatio...