A 6-week-old female infant presented with pallor, hepatosplenomegaly and a leucocyte count of 329 9 10/l (91% blasts). Light microscopy of the peripheral blood film showed blasts with rounded nuclei and scanty cytoplasm. No intracytoplasmic vacuoles were present (top left). Immunophenotyping by flow cytometry using peripheral blood (bottom panels) showed expression of CD10, surface membrane imm...

Five patients, four women and one man, age 32-8- yr, all whites, had refractory anemia with the same abnormal bone marrow karyotype, i.e., a partial deletion of the long arm of the No. 5 chromosome. The hematologic syndrome was practically the same in these five cases. Examination of the blood revealed a moderate to severe, generally macrocytic anemia with slight leukopenia but normal or elevat...

An asymmetric potato hybrid and its parental lines were cytogenetically examined. DAPI (4'-6-diamidino-2-phenylindole) staining was used to count chromosomes in all analysed lines and revealed the presence of minichromosomes in the hybrid genome. Fluorescent in situ hybridization (FISH) with rDNA sequence as a probe helped to determine the ploidy level of analysed lines and revealed that none o...

BACKGROUND AND AIMS Since the advent of molecular phylogenetics, numerous attempts have been made to infer the evolutionary trajectories of chromosome numbers on DNA phylogenies. Ideally, such inferences should be evaluated against cytogenetic data. Towards this goal, we carried out phylogenetic modelling of chromosome number change and fluorescence in situ hybridization (FISH) in a medium size...

MLL gene rearrangements are associated with an extremely poor prognosis in infants with acute lymphoblastic leukemia (ALL), but little is known about their clinical significance in older children. Therefore, we studied 45 cases of childhood ALL with abnormalities of chromosome 11q23 for rearrangement of the MLL gene to determine if this feature confers a uniformly poor prognosis. MLL gene rearr...

Pleural effusion in chronic myeloid leukemia (CML) is poorly understood and rarely reported in the literature. When the pleural effusion is caused by leukemic pleural infiltration, the differential white blood cell count of the effusion is identical to that of the peripheral blood, and the fluid cytology reveals leukemic blasts. We report here a case of bilateral pleural involvement of atypical...

T-cell prolymphocytic leukemia (T-PLL) is a rare and aggressive subtype of chronic lymphocytic leukemia. Usually it presents in older people with a median age of 61 years. T-PLL is characterized by elevated white blood cell (WBC) count with anemia and thrombocytopenia, hepatosplenomegaly, and lymphadenopathy; less common findings are skin infiltration and pleural effusions. The most frequent ch...

The genetic basis of hybrid male sterility in house mice is complex, highly polygenic, and strongly X linked. Previous work suggested that there might be interactions between the Mus musculus musculus X and the M. m. domesticus Y with a large negative effect on sperm head morphology in hybrid males with an F1 autosomal background. To test this, we introgressed the M. m. domesticus Y onto a M. m...

Beef cattle have been selected in particular for increased growth rates and greater muscle development. The myostatin gene is responsible for the muscle hyperplasia in double muscled animals and it is also responsible in these animals for differences, during foetal development, in the relative proportions of muscle fibre types. A mutation in the myostatin gene in Limousin cattle (F94L) has been...

The inflorescences of ten species of the genus Jatropha were fixed in Cornoy’s fluid (6:3:1). Acetocarmine stain (2%) was used for staining the pollen mother cells. Seven species exhibited 11 bivalents and 2n =22 and x=11. But the two other species, J.villosa var. villosa and J.villosa var. ramnadensis showed only 10 bivalents and 2n number of 20 chromosomes and x=10. The study concluded the oc...