To a paper on a new method for staining tubercle bacilli with Nachtblau which the writer published in 1941, Reenstierna added a short note (1), together with a plate, on its use in staining leprosy bacilli in nasal smears, films of blood, and sections of lepromata. As was the case with tubercle bacilli, the bacilli of leprosy were found to take a dark-blue color, to appear larger and more disti...

The following method is recommended for the staining of Corynebacterium diphtheriae. It provides a good contrast between the body of the organism and the granules in it. Cover the fixed and dried film with Loeffler's methylene blue, leave for five minutes, wash with water, decolorize rapidly with 1/1000 sulfuric acid. The acid should not stay on the film for more than a few seconds, wash with w...

Objective(s): The aim of this study was to investigate the effect of curcumin on the osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and its underlying potential mechanism.Materials and Methods: The tissue explant adherence method was used to isolate hPDLSCs. Flowcytometry, Alizarin Red staining and Oil Red ...

the present study was carried out for evaluation of calcoflour white staining (cws) as a rapid method for detection of pneumocystis carinii in respiratory specimens of rats as an animal model for human infection. a total of 35 spraque – dawley rats were divided into two groups. group1 (20 rats) received increasing doses of dexamethasone subcutaneously, and group 2(15 rats)as control group that ...

BACKGROUND Detection of C4d along peritubular capillaries (PTC) in renal allograft biopsies is an independent prognostic marker of poor long-term graft survival. It is typically associated with circulating donor-specific antibodies. Since only little information is available on the best technique to stain C4d, we compared the two methods most often used for detecting C4d in renal allograft spec...