BACKGROUND We evaluated the performance of three chromogenic media (Brilliance agar I [Oxoid, UK], Brilliance agar II [Oxoid], and ChromID MRSA [Biomérieux, France]) combined with broth enrichment and the Xpert MRSA assay for screening of methicillin-resistant Staphylococcus aureus (MRSA). METHODS We obtained 401 pairs of duplicate nasal swabs from 321 patients. One swab was suspended overnig...

A number of Escherichia coli clones were isolated from a Lactobacillus delbrueckii subsp. lactis gene library capable of hydrolysing the chromogenic substrate Gly-Ala-beta-naphthylamide (Gly-Ala-beta NA). Some of the recombinant plasmids carried by these clones have been shown to encode the cysteine aminopeptidase gene pepC. Nucleotide sequence analyses of the plasmid inserts of the remaining c...

A new dual-ion-switched molecular brake was synthesized and operated by chemical reagents (H+/OH- and Zn2+/EDTA), and its rotation could be read out by chromogenic, fluorogenic and electrochemical signals.

The chromogenic Limulus amoebocyte lysate test effectively detected 66 (100%) culture-proven gram-negative peritonitis cases among 185 continuous ambulatory peritoneal dialysis patients with clinical evidence of infectious peritonitis.

The different optical responses in chromogenic sensor stimulated by addition of Cu(2+) (red to blue) and Ni(2+) (red to green) allow simultaneous estimation of Cu(2+) and Ni(2+).

A new naked-eye chromogenic chemosensor based on 2-thiohydantoin shows high selectivity for fluoride ions and is used to develop a test paper for detection of fluoride ions in the solid state.

The highly selective chemodosimetric behavior of thiocoumarins toward Hg(2+) ions was investigated; significant chromogenic and fluorogenic signaling of Hg(2+) ions occurs from the transformation of thiocoumarin to coumarin by Hg(2+)-induced selective desulfurization.

Four methods (chromogenic, acidimetric, inhibition, and iodometric) for demonstration of the beta-lactamase production by 70 isolates of Neisseria gonorrhoeae, were evaluated in Cuba. There was 100% correlation between all beta-lactamase methods and the standardized penicillin dilution susceptibility test for penicillinase-non-producing N. gonorrhoeae. For penicillinase-producing N. gonorrhoeae...

BACKGROUND Current coagulation tests lack sensitivity and detect disseminated intravascular coagulation (DIC) only when it is severe. Measurement of antithrombin (AT) activity and D-dimer concentration permits early diagnosis and more precise classification of coagulopathies in some species. OBJECTIVES The objectives of this study were to validate and determine the diagnostic utility of a chr...

Background: Subtilisin –like proteases are the group of proteases including keratinases found in dermatophytes which degraded keratin. Determination of the proteases activity of Trichophyton vanbreuseghemii isolates which were obtained from soil and clinical and soil isolates of Microsporum gypseum in Iran and characterization of their genome were aim of present study. Methods: Ezymatic activit...