Temperature-Controlled Microchip Liquid Chromatography System Thesis by Victor Chi-Yuan Shih Doctor of Philosophy in Electrical Engineering California Institute of Technology High-performance liquid chromatography (HPLC) is one of the most important analytical tools heavily used in the fields of chemistry, biotechnology, pharmaceutics, and the food industry. The power of liquid chromatography c...

In the field of stabilizer analysis today there is a need for the analytical determination of the decomposition or transformation products of the stabilizers in polymer systems including their reaction products with the polymers. For the solution of such a complex analytical task, separation problems are at the centre of modern scientific work. These separation problems are subdivided into two ...

introduction: polyclonal antibodies are required to be affinity purified. improved purification methods of polyclonal antibody provide an opportunity to pick the most purified immunoglobulins as a primary or secondary antibody in immunoassays that are included in many vaccine studies. two common techniques for purifying proteins is salt precipitation and chromatography purification. our work fo...

Computer simulation was used to optimize the separation of a tryptic digest of recombinant human growth hormone using reversed-phase high-performance liquid chromatography in a gradient mode. DryLab G/plus software modelled the retention behavior of the complex tryptic digest mixture as a function of gradient conditions, based on data from two experimental gradient runs. The theoretical optimum...

Selection of mobile phases markedly affect separation efficiency of eluted probes in gas chromatography (GC), the mobile phases have a pronounced effect on the retention times and efficiency of natural gas separation. In solid chromatography, carrier gases which are adsorbed to any extent occupy more active adsorption sites, reducing the net heat of adsorption of the eluted zone. The efficiency...

Background: Plasminogen is one of the compounds derived from human plasma. Activation of plasminogen produces plasmin. Plasmin is able to lyse fibrinogen, fibrin, and some other human plasma proteins. The aim of the present work was to study the separation of human plasminogen by affinity chromatography using gel lysine Sepharose. Materials and Methods: Normal human plasma was used as the...

In this study, a new silica gel based adsorbent was fabricated and its ability in separation and quantification of alkanes mixture was investigated. Silica gel (SiO2) is a polar absorbent which is mainly used to separate polar compounds. Also, the carbon materials such as activated carbon and recently carbon nanotube (CNTs), have been widely used for separation of nonpolar materials. Carbon nan...