In this study chromatographic behavior of mouse serum immunoglobulin G (IgG) on a protein G perfusion affinity chromatographic column was investigated experimentally. The results indicate that the protein G column has no non-specific binding to the other proteins in mouse serum but an irreversible adsorption to IgG under the conditions investigated. It was found that variations of the elution s...

This review focuses on the recent advances on microscale separation technology, particularly on the new development on column and detection technologies in electrophoretic and chromatographic separation modes (i.e., CE, CEC, cLC, etc.). Representative examples illustrate also applications on the small molecules, peptides, proteins, polysaccharides, chiral and so on. 246 relevant articles are ci...

A fast method to obtain a quantitative structure-retention relationship is required in chromatography for the rapid optimization of chromatographic separation conditions. Chromatographic data of acidic drugs are analyzed by a computational chemical method to simulate chromatographic simulation. The direct interaction between a model phase and a drug is calculated as an energy value using the mo...

This paper reviews recently published chromatographic methods for the analysis of plant sterols in various sample matrices with emphasis on vegetable oils. An overview of structural complexities and biological/nutritional aspects including hypocholesterolemic activities of phytosterols is provided in the Section 1. The principal themes of the review highlight the development and application of ...

This Technical Overview demonstrates and discusses the influence of the injection parameters Feed Speed and Overfeed Volume on chromatographic separation. These parameters were introduced with the Agilent 1260 Infinity II Supercritical Fluid Chromatography (SFC) Multisampler. The influence on the isocratic separation of closely eluting compounds, shown for different injection volumes commonly u...

This paper describes fundamentals and applications of multivariate statistical techniques for the optimization of chromatographic systems. The surface response methodologies: central composite design, Doehlert matrix and Box-Behnken design are discussed and applications of these techniques for optimization of sample preparation steps (extractions) and determination of experimental conditions fo...

This paper presents a novel stationary phase for silicon microfabricated columns for gas chromatographic (GC) separation of light alkanes. Coated columns show an excellent efficiency, up to 8000 theoretical plates per meter and enable complete separation of light isomeric alkanes. An optimized column, with a thicker deposition, even enables the fast separation of a natural gas like mixture of l...

Computer-assisted optimization of chromatographic separations is still a fruitful activity. In fact, advances in computerized data handling should make the application of systematic optimization strategies much easier. However, in most contemporary applications, the optimization criterion is not considered to be a key issue (Vanbel, J Pharm Biomed, 21:603-610, 1999). In this paper, an update of...

L-Thyroxine (L-T4), the naturally occurring thyroid hormone has been used for the treatment of thyroid dysfunctions, while D-T4 is not active in the thyroid and is used for the treatment of hyperlipidemia. Therefore, a convenient and reliable enantiomer separation method has been of great interest in the biological and pharmacological research field. Several groups reported the chromatographic ...

A new chiral liquid-chromatographic method was developed for the enantiomeric separation of ofloxacin with a fluorescence detector. The enantiomers of ofloxacin were baseline resolved on a Chiralcel OD-H (250 mm x 4.6 mm, 5 microm) column using a mobile-phase system containing hexane-ethanol-methanol-acetic acid-diethylamine (70/20/10/0.45/0.05, v/v/v/v/v). The presence of diethylamine in the m...