Abstract Infiltrating neutrophils and neutrophil extracellular traps (NETs) accumulate in the inflamed epithelium to induce mucosal inflammation patients with inflammatory bowel diseases. The formation of NETs is initiated by peptidyl arginine deiminase 4 (PAD4), which facilitates NET generation through chromatin decondensation. We recently demonstrated that impair intestinal integrity, permeab...

Cellular DNA is constantly challenged by damage-inducing factors derived from exogenous or endogenous sources. In order to maintain genome stability and integrity, cells have evolved a wide variety of DNA repair pathways which counteract different types of DNA lesions, also referred to as the DNA damage response (DDR). However, DNA in eukaryotes is highly organized and compacted into chromatin ...

Solubilization of cross-linked cells followed by chromatin shearing is essential for successful chromatin immunoprecipitation (ChIP). However, this task, typically accomplished by ultrasound treatment, may often become a pitfall of the process, due to inconsistent results obtained between different experiments under seemingly identical conditions. To address this issue we systematically studied...

BACKGROUND Currently, relation between reactive oxygen species (ROS) ROS concentration and semen quality was indicated. Saffron has traditionally been not only considered as a food additive but also as a medicinal herb, which has a good antioxidant properties. OBJECTIVE The aim of this study was to evaluate the protection potency of saffron and vitamin E on sperm chromatin integrity. MATERI...

During the last decade, chromatin research has been focusing on the role of histone variability as a modulator of chromatin structure and function. Histone variability can be the result of either post-translational modifications or intrinsic variation at the primary structure level: histone variants. In this review, we center our attention on one of the most extensively characterized of such hi...

in this study the effect of a methylated derivative of nitrogen mustard (mechloroethamin) on solubilized and reconstituted chromatin was investigated using uvnis spectroscopy, dna and sds gel electrophoresis and densitomeoic scanning techniques, the results indicated that the 210 and 260 nm absorbances of reconstituted chromatin were decreased in a dose-dependent manner after treatment with var...