background: toxoplasma gondii can infect all warm-blooded animals. modified agglutination test (mat) and elisa are widely used for the detection of t. gondii antibodies. however, there is little information on their acceptability for detecting antibodies in companion animals. methods: this study compared elisa and mat for their ability to detect t. gondii infection in natu­rally infected dogs a...

From fruiting bodies of L. edodes strain L-54, single-spore isolates (SSIs) were collected. Two parental types of L-54 were regenerated via monokaryotization. By means of random-amplified polymorphic DNA (RAPD), DNA samples from L-54, its two parental types, and 32 SSIs were amplified with arbitrary primers. Dedikaryotization was demonstrated, and 91 RAPD-based molecular markers were generated....

The heterothallic fungus Podospora anserina has two mating-type alleles termed mat+ and mat-. The mat+ sequence contains one gene, FPR1, while mat- contains three genes: FMR1, SMR1, and SMR2. FPR1 and FMR1 are required for fertilization, which is followed by mitotic divisions of the two parental nuclei inside the female organ. This leads to the formation of plurinucleate cells containing a mixt...

to evaluate fertility status and mating type distribution of cryphonectria parasitica, causal agent of chestnut blight in guilan province, four sites in two main growing regions of: shaft (visrud, taleghan and babarekab), and rezvanshahr (doran) were investigated. during the study, fifty four isolates of c. parasitica were examined. c. parasitica isolates were crossed with each of the two matin...

Metabolic alteration is a hallmark of cancer. Dysregulation of methionine metabolism is implicated in human liver cancer. Methionine adenosyltransferase IIα (MAT IIα) is a key enzyme in the methionine cycle, catalysing the production of S-adenosylmethionine (SAM), a key methyl donor in cellular processes, and is associated with uncontrolled cell proliferation in cancer. Here we show that P300 a...

This paper presents a real time force sensing instrument that is designed for human gait analysis purposes. It is capable of recording and monitoring ground reaction forces exerted by human foot during various activities such as walking, running and jumping in real time. In overall, force sensing mat mainly consists of three elements: the force sensing mat, signal conditioning circuit and data ...

In this study, the occurrence of antibodies to Toxoplasma gondii in Brazilian agouti (Dasyprocta aguti) was compared by modified agglutination test (MAT) and indirect fluorescent antibody test (IFAT) using anti-capybara conjugate. Sera from 109 animals were tested using MAT (1:25 cut-off) and IFAT (1:16 cut-off); 19% were positive by MAT, and 18% were positive by IFAT. Overall, the 17 IFAT-posi...

This work presents an insight into the effect of conductive filler content on both electrical and thermal properties of a polymer composite system. The electrical conductivity of an insulating polymer can be achieved by dispersing conducting fillers (e.g., metal, graphite powder, carbon black, carbon fiber) in the polymer matrix. The resulting materials are referred to as conducting polymer com...

Studies of heterothallic and homothallic strains of Saccharomyces cerevisiae have led to the suggestion that mating-type information is located at three distinct sites on chromosome 3, although only information at the mating-type (MAT) locus is expressed (Hicks, Strathern and Herskowitz, 1977). We have found that the recessive mutation cmt permits expression of the normally silent copies of mat...