Microalgae have increasingly gained research interest as a source of lipids for biodiesel production. The wet way processing of harvested microalgae was suggested and evaluated with respect to the possible environmental impacts and production costs. This study is focused on the three key steps of the suggested process: flocculation, water recycling, and extraction of lipids. Microalgae strains ...

a series of non-cyclic polyethers with different end groups and chain length were used for the extraction of lithium salt from aqueous into various organic phases viz. chloroform, carbon tetrachloride, 1,2-dichloroethane, to study the influence of structural variations within the extractant molecule upon the extraction selectivity and efficiency. the ionophores used were viz. deg (i), degdme (i...

Membrane proteins are involved in diverse cellular processes and are an integral component of many signaling cascades, but due to their highly hydrophobic nature and the complexities associated with studying these proteins in planta, alternative methods are being developed to better characterize these proteins on a proteome-wide scale. In our previous work (Mitra, S. K.et al. J. Proteome Res. 2...

An easy and rapid protocol to extract DNA to be used as template for polymerase chain reaction (PCR) fingerprinting experiments from cultivable lactic acid bacteria (LAB) is proposed. Different procedures for rapid extraction of DNA by chelex (iminodiacetid acid) ionic resin were compared. Factors affecting the quality and reproducibility of PCR fingerprinting profiles were also investigated. T...

The recovery of phospholipid, ester-linked fatty acids and phospholipid phosphate from the eubacterium Pseudomonas atlantica was shown to be equivalent for the modified Bligh and Dyer (chloroform/methanol/water) procedure and a hexane/isopropanol (HIP) lipid extraction. In addition to its previously suggested lower toxicity, the HIP solvent system allows lipid analyses to be routinely done on c...

regarding increasing rate of drug resistance among microbial pathogens, a global search to find new antimicrobial agents from natural compounds with fewer side effects has been considered by many researchers worldwide. bioactive compounds with good antimicrobial activity have been isolated from different algae and cyanobacteria. the current study was performed to determine antimicrobial potenti...

In this study, optimization is carried out to find the optimized design of a foam-filled column for the best Specific Energy Absorption (SEA) and Crush Force Efficiency (CFE). In order to maximize SEA, the optimization gives the value of 2.3 for column thickness and 151.7 for foam length. On the other hand to maximize CFE, the optimization gives the value of 1.1 for column thickness and 200 for...

Chlorophyll analysis at high precision and accuracy is limited by the lack of suitable, commercially available internal standards for HPLC analysis. Here, the commercially available dye zinc-phthalocyanine is presented as a new internal standard to quantify chlorophylls in vegetable foods and to detect chlorophyll degradation products. The technique was applied to chlorophyll analysis of a sele...

This work represents an application of the Visible Spectrometry, High Pressure Liquid Cromatography and Electronspray Ionisation-Mass Spectrometry analysis for the identification of chlorophyll and its derivatives, pheophytin and chlorophyllide, in their purified mixtures (the chlorophyll, pheophytin and chlorophyllide fraction, respectively). The chlorophyll, pheophytin and chlorophyllide frac...

Dispersive liquid liquid microextraction (DLLME) combined with gas chromatography–mass spectrometry (GC–MS) was used for the extraction and determination of 13 polycyclic aromatic hydrocarbons (PAHs) in mineral water samples. In this procedure, the suitable combination of extraction solvent (500 µL chloroform) and disperser solvent (1000 µL acetone) were quickly injected into the water sample (...