The major light-harvesting chlorophyll-a/b complex in most higher plants contains three carotenoids, lutein, neoxanthin, and violaxanthin. How these pigments are assembled into the complex during its biogenesis is largely unknown. Here we show that neoxanthin but not lutein can dissociate from the fully assembled complex. Its equilibrium binding constant in a detergent system (0.1% n-dodecyl-be...

The primary electron donor P700 in photosystem I is composed of two chlorophylls, P(A) and P(B). P700 forms the cationic [P(A)/P(B)](•+) state as a result of light-induced electron transfer. We obtained a P(A)(•+)/P(B)(•+) ratio of 28:72 and a spin distribution of 22:78 for the entire PSI protein-pigment complex. By considering the influence of the protein components on the redox potential for ...

HPLC analysis of pigments of the symbiotic algae of the coral Goniastl-ea aspera (Verrill) showed the presence of the chlorophylls a and c2 (chl a and c,) and the carotenoids pendlnin, diadinoxanthin, diatoxanthin and p-carotene. Increased production of diatoxanthin was seen In algae extracted from the western surfaces of corals, exposed to high levels of solar radiation, during a solar bleachi...

Photosystem II Herbicides, Chlorotic Herbicides, Chlorophylls, Carotenoids, Phytochrome, Inhibitors Etioplasts of radish seedlings treated with photosystem II (DCMU, bentazon) and chlorotic herbicides (amitrole, SAN 6706) were tested on their ability to perform the phytochrome mediated chlorophyll and carotenoid biosynthesis. The cytoplasmic influence on the chloroplastic action of herbicides w...

Peridinin-chlorophyll-protein, a water-soluble light-harvesting complex that has a blue-green absorbing carotenoid as its main pigment, is present in most photosynthetic dinoflagellates. Its high-resolution (2.0 angstrom) x-ray structure reveals a noncrystallographic trimer in which each polypeptide contains an unusual jellyroll fold of the alpha-helical amino- and carboxyl-terminal domains. Th...

Peripheral magnesium complexes of methyl pheophorbides a and b, bacteriomethyl pheophorbide a, bacteriopheophytin a, and protopheophytin a have been prepared and characterized by UV-vis, IR, ‘H N M R , and luminescence spectroscopy. In these compounds, Mg is bound to the peripheral p-keto ester system present in most chlorophylls rather than to the central tetrapyrrole cavity as in the chloroph...

Pigments were monitored in parsley leaves stored in air, air + 10 ppm GH.,, or 10% O2 + 10% CO2 controlled atmosphere (CA). Chlorophylls a and b, as determined with HPLC, decreased sharply in leaves held in air or air + 10 ppm GH4. The decrease was less in leaves held in 10% O2 and 10% CO2 CA. The oxidized product of chlorophyll a, lo-hydroxychlorophyll a, did not accumulate and chlorophyllide ...

The study of the pigment apparatus (chlorophylls, carotenoids) and flavonoids will allow to reveal plants adaptation mechanisms, which subsequently can be used in ecological biochemical studies. purpose is determine content photosynthesis pigments phytomass hydroponically grown garden sage (Salvia officinalis L.) compared with open soil. Two varieties were studied: ‘Dobrinya’ ‘Kubanets’ from tw...

Chloroplast pigments from young and old leaves of three chlorophyll-deficient peanut mutants (lutescens, auredus, and virescent) and two "normal" peanut genotypes ('Chico' and 'Guanajuato') were analyzed using high performance liquid chromatography. All genotypes showed chlorophylls a and b, carotene, lutein, neoxanthin, and violaxanthin in young and old leaves. Generally, there were decreases ...