THE EFFECT OF EXPOSING RABBIT RETICULOCYTE RIBOSOMES TO CONCENTRATED SOLUTIONS OF POTASSIUM CHLORIDE WAS EXAMINED BY: (a) dialysis against 0.5m-potassium chloride; (b) zone centrifugation through a sucrose gradient in 0.5m-potassium chloride; (c) differential centrifugation of a solution made 0.5m with respect to potassium chloride. The products of each treatment and their ability to support pr...

Gill Na(+)/K(+)-ATPase (NKA) in teleost fishes is involved in ion regulation in both freshwater and seawater. We have developed and validated rabbit polyclonal antibodies specific to the NKA alpha1a and alpha1b protein isoforms of Atlantic salmon (Salmo salar Linnaeus), and used western blots and immunohistochemistry to characterize their size, abundance and localization. The relative molecular...

The GABA responses of fast-spiking (FS) interneurons and regular-spiking (RS) principal cells were studied using whole cell and perforated-patch recordings in slices of the basolateral amygdala, neo-, and perirhinal cortex. In these three areas, responses to exogenous and synaptically released GABA were abolished by GABA(A) receptor antagonists in FS cells but also included a GABA(B) component ...

Calcium-activated chloride channels of the anoctamin (alias TMEM16) protein family fulfill critical functions in epithelial fluid transport, smooth muscle contraction and sensory signal processing. Little is known, however, about their contribution to information processing in the central nervous system. Here we examined the recent finding that a calcium-dependent chloride conductance impacts o...

The reaction of barium chloride with 2, 6-pyridinedicarboxylic acid in water led to the formation of a novelpolymeric complex formulated as {[Ba2(Hpydc)2(pydc)(H20)3].H20.(H2pydc)}.(I). The crystal structure of (I)was characterized by single crystal X-ray diffraction method. The complex was crystallized in the monoclinicsystem with space group P2/c, with four molecule in unit-cell, e.g Z=4. The...

Remarkable activity-dependence was uncovered in the chloride conductance that operates in the subthreshold region of membrane potential, by using the twomicroelectrode voltage-clamp technique in the mature and intact rat sympathetic neuron. Both direct and synaptic neuron tetanization (15 Hz, 10 s duration to saturate the response) resulted in a long-lasting (not less than 15 min) increase of c...

Vasoactive intestinal polypeptide (VIP) and the calcium ionophore A23187 caused dose-dependent changes in the potential difference and the short circuit current (Isc) across confluent T84 cell monolayers mounted in modified Ussing chambers. Both VIP and A23187 stimulated net chloride secretion without altering sodium transport. Net chloride secretion accounted for the increase in Isc. When A231...

The study was undertaken to investigate the possible correlation of total volume of mitochondria per cell with the rate of succinate oxidation in isolated nerve cell bodies, after various functional stresses in the experimental animals. Significant cytological effects were found in the nerve cells of the supraoptic nucleus in rats which had been thirsting for 4-12 days or had been given 2% sodi...