The mammalian oocyte has played a prominent role in the development of the science of Cryobiology. Following up on the success of spermatozoa cryopreservation in the late 1940's, attempts to cryopreserve rabbit oocytes using glycerol as the cryoprotectant were undertaken. Unfortunately, the outcomes of these early studies were rather disappointing. The recalcitrance of mammalian oocytes to cryo...

The effects of chilling on the photosynthesis of a chilling-resistant species, pea (Pisum sativum L. cv Alaska) and a chilling-sensitive species, cucumber (Cucumis sativus L. cv Ashley) were compared in order to determine the differences in the photosynthetic chilling sensitivity of these two species. For these experiments, plants were chilled (5 degrees C) for different lengths of time in the ...

The effect of rapid chilling of pork carcasses on bacterial populations was examined. Two regimes of rapid chilling were applied: treatment 1 (at –31°C in the first 3 h of chilling, and then at 2–4°C till 8 h post-mortem) and treatment 2 (at –31°C in the first 3 h of chilling, and then at 2–4°C till 24 h post-mortem). Microbiological sampling of pork carcasses was obtained by swabbing. Swab sam...

Methyl salicylate (MeSA) vapor increased resistance against chilling injury (CI) in freshly harvested pink tomatoes. The expression patterns of alternative oxidase (AOX) before and during the chilling period demonstrated that pre-treatment of tomato fruit with MeSA vapor increased the transcript levels of AOX. We used 4 EST tomato clones of AOX from the public database that belong to two distin...

Postharvest chilling injury (PCI) is a physiological disorder that contributes to the global loss and waste of fruit vegetables. Ectopic expression C-Repeat Binding Factor (CBF) transcription factors in tomato (Solanum lycopersicum L.) alleviates vegetative injury, but their influence on PCI unknown. We developed dexamethasone (DEX)-inducible system with aim limiting high ectopic Arabidopsis th...

The regulatory metabolite, fructose 2,6-bisphosphate (Fru 2,6-P(2)) was found in green pepper (Capsicum annuum L.). The Fru 2,6-P(2) level was found to: (a) rise rapidly in response to heat; (b) drop rapidly, followed by recovery, in response to cold storage of fruit and, (c) oscillate during cold storage of fruit. The possible existence of a relationship between chilling injury and Fru 2,6-P(2...

Direct water immersion chilling is used widely in the poultry plants because of higher heat transfer rate. However, post-mortem state of poultry muscle, extent of water uptake by carcasses prior to deboning, and marination has been one of the largest sources of inconsistency in marinated product quality and has become a problem for producers of marinated broiler meat. The effect of Ultra-rapid ...

Insufficient chilling resulting from rising winter temperatures associated with climate warming has been an area of particular interest in boreal and temperate regions where a period of cool temperatures in fall and winter is required to break plant dormancy. In this study, we examined the budburst and growth of trembling aspen (Populus tremuloides Michx.), balsam poplar (Populus balsamifera L....