In mammals, the inducible cytokine interleukin 10 is a feedback negative regulator of inflammation. To determine the extent to which this function is conserved in birds, recombinant chicken IL-10 was expressed as a secreted human Ig Fc fusion protein (chIL-10-Fc) and used to immunise mice. Five monoclonal antibodies (mAb) which specifically recognise chicken IL-10 were generated and characteris...

This paper describes the biochemical characterization of the chicken oocyte plasma-membrane receptor for one of the major lipid-carrying yolk proteins, vitellogenin (VTG). The receptor was extracted from oocyte membranes with the non-ionic detergent octyl-beta-D-glucoside and visualized by ligand blotting, with 125I-VTG as a protein with an apparent Mr of 96000, under non-reducing conditions. I...

BACKGROUND Antibody fragments selected from large combinatorial libraries have numerous applications in diagnosis and therapy. Most existing antibody repertoires are derived from human immunoglobulin genes. Genes from other species can, however, also be used. Because of the way in which gene conversion introduces diversity, the naïve antibody repertoire of the chicken can easily be accessed usi...

Membrane extracts from chicken smooth muscle contain, along with filamin, vinculin and alpha actinin, a group of polypeptides that have the ability to interact with the "barbed end" of actin filaments. These low molecular mass polypeptides were designated as HA1 (Wilkins, J.A., and S. Lin, 1986, J. Cell Biol., 102:1085-1092). In this study, polyclonal antibodies raised against the HA1 preparati...

Neospora caninum is an obligate intracellular protozoan that causes abortion and economic loss in the cattle industry. This study aimed to estimate the prevalence of anti-N. caninum antibodies in chicken using ELISA methods based on the surface antigen 1 of N. caninum (NcSAG1t). The overall prevalence of N. caninum in chicken was 15.51%. The seroprevalence was high in Qalyoubiya, Minufiya, Kafr...

It has been demonstrated that embryonic chicken gizzard smooth muscle contains a unique embryonic myosin light chain of 23,000 mol wt, called L23 (Katoh, N., and S. Kubo, 1978, Biochem. Biophys. Acta, 535:401-411; Takano-Ohmuro, H., T. Obinata, T. Mikawa, and T. Masaki, 1983, J. Biochem. (Tokyo), 93:903-908). When we examined myosins in developing chicken ventricular and pectoralis muscles by t...

1164, Date 1:00 pm, Sunday, February 11, 2007 (24 hours) Session D1: Poster Preliminary Characterization of Anti-WDR1 Antibody in the Avian Inner Ear *Henry J. Adler, Elena Sanovich, Kai Yan, Robert J. Dooling Studies on hearing loss and recovery in birds and mammals are now identifying, within the ear, protein changes that lead to functional disability. One of the proteins, WD repeat-1 protein...

The study was conducted to know the rate of maternally derived antibodies (MDAs) transfer from parents to their offspring and declining the MDAs in their chicks at 0, 7, 14, and 21 days of age against four major poultry viruses like Newcastle disease virus (NDV), Infectious bronchitis virus (IBV), Infectious bursal diseases virus (IBDV), and Avian Reo virus (ARV). The MDAs was studied on Grandp...

The stabilization of antibody-latex complexes at high salt concentration is an event that cannot be explained by the widespread DLVO theory. Adsorption of antibodies on polystyrene latex usually leads to a loss in colloidal stability. However, after the expected particle aggregation induced by an increase in ionic strength, an 'anomalous' restabilization occurs when the electrolyte concentratio...

It is commonly established that warm blooded animals are extremely vulnerable to the infectious coccidian parasite, Toxoplasma gondii. T. gondii can be transmitted poultry and livestock through ingestion of oocyst (fecal-oral), placenta (congenital toxoplasmosis) in livestock. Cattle, sheep, rabbits some most consumed Palestine. As for poultry, backyard chicken, caged turkeys birds. The aim pre...